摘要
目的:评价神经干细胞与胶原蛋白-硫酸肝素支架的生物相容性,探讨胶原蛋白-硫酸肝素支架作为中枢神经组织工程载体材料的可行性。方法:以冷冻干燥法制备胶原蛋白-硫酸肝素支架,体外培养新生小鼠海马神经干细胞。将神经干细胞与生物支架共培养,通过倒置相差显微镜、扫描电镜观察胶原蛋白-硫酸肝素支架内部结构和细胞生长状况。结果:制备的胶原蛋白-硫酸肝素支架材料具有纵行的、平行排列的微管结构。神经干细胞在支架上生长良好。结论:胶原蛋白-硫酸肝素支架与神经干细胞具有良好的生物相容性,有望作为中枢神经组织工程载体材料。
Objective: To evaluate the biocompatibility of neural stem cells (NSCs) with collagen protein-heparan sulfate scaffold in vitro and to identify whether the materials could be used as scaffold in the central neural tissue engineering. Method: The collagen protein-heparan sulfate scaffold was made by frozen-lyophilized. Hippocampusderived NSCs of neonatal mouse were cultured and combined with scaffold biomaterials in vitro. The microstructure of the material and the morphological changes of NSCs in the scaffold were observed under an inverted phase microscope (IPM) and scanning electron microscope (SEM). Result: The microtubes of scaffold were arranged in vertical and parallel manners and their three-dimensional structures were highly bionic. The NSCs could regenerate and differentiate in the scaffold in vitro. Conclusion: The scaffold has a good biocompatibility with NSCs and might be used as biological materials in central nervous tissue engineering.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2008年第2期135-137,I0001,共4页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金资助项目(30570628,30770751)
华中科技大学医工医理交叉基金资助项目
关键词
神经干细胞
生物材料
胶原蛋白
硫酸肝素
神经组织工程
neural stem cells
biological material
collagen protein
heparan sulfate
nervous tissue engineering