摘要
八氢番茄红素合酶(phytoene synthase,PSY)是类胡萝卜素生物合成的限速酶,通过建立psy基因的人参转化体系,可促进相应类胡萝卜素的合成,从而提高人参的营养价值。以人参愈伤组织为受体,以psy为目的基因,应用根癌农杆菌介导法进行遗传转化。以抗性筛选人参受体转染效率为指标,从菌液浓度、侵染胞龄、侵染时间、共培养时间四方面优化了转化体系。进行了PCR,PCR-Southern和RT-PCR分析鉴定及β-胡萝卜素含量测定,初步证明外源基因psy已整合到人参的基因组中并在转录水平上进行了表达,β-胡萝卜素含量平均提高了26倍。为改善和提高人参中类胡萝卜素含量提供了一种新的途径。
Phytoene synthase is a rate-limiting enzyme in the pathway of carotenoid biosynthesis. To aim at establishing a transformation of Ginseng callus cells, elevating the nutritive value through encouraging the composition of corresponding carotenoid, taking Ginseng callus cells as acceptor,psy gene were transformed into cells via Agrobacterium-mediated. The factors affecting genetict ransformation were also investigated seperately from concentrations of A. tumefaciens, age of Ginseng callus cells, infection time and co-culture time. PCR,PCR -Southern and RT-PCR analysis from the transferred gene plants proved that psy gene has been transferred into Ginseng callus cells and can be expressed. The content of β-carotene was increased by an average of 26 times. A base of improving and raising the contents of carotenoid in the Ginseng callus cell was established.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第2期90-95,共6页
China Biotechnology
基金
吉林省教育厅资助项目(JYT200608)
关键词
农杆菌法
psy基因
人参愈伤组织
遗传转化
Agrobacterium tumefaciens psy gene Ginseng callus cells Genetic transformat
