摘要
目的:探讨益气化瘀中药对糖尿病皮肤溃疡气虚血瘀证大鼠缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)和血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的影响。方法:72只清洁级雄性SD大鼠,除8只作正常对照组外,其他大鼠在背部全层皮肤缺损开放性创面的基础上加造气虚血瘀证糖尿病模型后,随机分为模型组、贝复济组、益气化瘀组、益气组和化瘀组。应用免疫组织化学、图像分析等技术,观察各组大鼠创面肉芽组织中HIF-1α和VEGF水平的变化。结果:模型组HIF-1α水平高于正常对照组(P<0.01),VEGF浓度明显低于正常对照组(P<0.01)。各用药组HIF-1α表达水平明显低于模型组(P<0.01),VEGF表达水平明显高于模型组(P<0.01)。益气化瘀组HIF-1α表达水平明显低于贝复济组、益气组和化瘀组(P<0.05或P<0.01),VEGF表达水平明显高于贝复济组(P<0.01)。益气组与化瘀组HIF-1α和VEGF的表达水平相似,它们与贝复济组之间无明显差异。结论:益气化瘀中药能明显促进糖尿病皮肤溃疡气虚血瘀证大鼠的创面愈合,其机制与下调HIF-1α水平、上调VEGF的表达水平和改善局部缺血缺氧状态有关。
Objective： To explore the effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1α （HIF-1α） and vascular endothelial growth factor （VEGF） in granulation tissue of skin ulcers in rats with syndrome of blood stasis and qi deficiency. Methods： Diabetic rats with back full-thickness skin lesion and syndrome of blood stasis and qi deficiency were divided in to five groups： untreated group, basic fibroblast growth factor （bFGF）-treated group, Yiqi Huayu Recipe （a recipe for replenishing qi and resolving stagnation）-treated group, Yiqi Recipe （a recipe for replenishing qi）-treated group and Huayu Recipe （a recipe for resolving stagnation）-treated group, and another eight normal rats served as normal control group. Immunohistochemical method and image analysis were used to test the expressions of HIF-1α and VEGF in granulation tissue of skin ulcers in rats with diabetes. Results： In the untreated group, the expression of HIF-1α was significantly increased and the expression of VEGF was significantly decreased as compared with those in the normal control group （P〈0.01）. The expression of HIF-1α was obviously lower （P〈0.01） and the expression of VEGF was significantly higher in the four drug treated groups as compared with those in the untreated group. In the Yiqi Huayu Recipe-treated group, the expression of HIF-1α was obviously lower than those in the bFGF-treated, Yiqi Recipe-treated and Huayu Recipe-treated groups （P〈0.05 or P〈0.01）, and the expression of VEGF was significantly higher than that in the bFGF-treated group （P〈0.01）. Conclusion： Chinese herbs for replenishing qi and resolving stagnation can promote the wound healing in rats through reducing the expression of HIF-1α, accelerating the expression of VEGF in granulation tissue of skin ulcers in rats with diabetes and ameliorating the status of ischemia and hypoxia.
出处
《中西医结合学报》
CAS
2007年第2期165-169,共5页
Journal of Chinese Integrative Medicine
基金
上海市重点学科建设基金资助项目(NoT0304)
上海市青年科技启明星培养计划资助项目(No03QB14047)
