摘要
为探索甲型肝炎减毒活疫苗的残余毒力及毒力返祖的可能性，将血清抗－ＨＡＶ阴性，肝功能正常的普通狨猴用于甲型肝炎减毒活疫苗Ｈ２株传代试验。即用甲型肝炎疫苗接种普通狨猴，将其粪便排出的子代病毒，直接用粪便悬液或／和用组织培养增殖液接种狨猴进行传代。３次连续体内传代的结果揭示：血清抗－ＨＡＶ无明显差异，没有或有低水平肝脏酶活性变化，全部肝脏组织检查无病理学改变，全部动物排毒，但排毒量少。
Common marmosets with negative serum antibodies against hepatitis A virus (anti HAV) and normal liver functions were used for the tests of passage of H 2 vaccine strain of HAV to explore its residual virulence after attenuation and possibility of its reversion. Common marmosets were inoculated with attenuated live vaccine, and fecal suspension and/or proliferative liquid in tissue culture of the progeny of vaccine HAV excreted from their feces were passaged in common marmosets. Result of three serial passages in vivo indicated that there was no significant difference in serum anti HAV titer, and no or low level changes in activities of liver enzymes, no pathological changes in liver tissues observed, and detectable but minimal HAV was shed in stool of the all animals.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1997年第5期260-262,共3页
Chinese Journal of Preventive Medicine
基金
国家"八.五"攻关课题
美国中华医学基金会(CMB)基金
