摘要
目的检测慢性肾衰竭（CRF）患者血清基质金属蛋白酶-9（MMP-9）及金属蛋白酶组织抑制因子-1（TIMP-1）表达状况，拟在探讨其与肾脏纤维化的关系。方法采用ELISA法测定60例CRF患者（氮质血症期15例，肾衰竭期24例，尿毒症终末期21例）和20例正常对照组血清MMP-9、TIMP-1水平，同时收集患者临床资料，分析血清MMP-9、TIMP-1水平和MMP-9／TIMP-1比值与肾功能损害等临床指标之间的相关性。结果CRF患者各期血清MMP-9、TIMP-1水平明显升高，MMP-9／TIMP-1比值显著降低，随着肾小球滤过率（GFR）下降，MMP-9、TIMP-1呈降低趋势，而MMP-9／TIMP-1比值却逐渐上升。血清TIMP-1水平和MMP-9／TIMP-1比值与血肌酐、GFR及血红蛋白呈高度相关性。结论血清MMP-9、TIMP-1参与了CRF的进展，血清TIMP-1水平或MMP-9／TIMP-1比值能较好地反映肾脏纤维化的程度。
Objective To determine the levels of serum metalloproteinase matrix -9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in cases with chronic renal failure（CRF）, and explore their relation with the renal fibrosis. Method The levels of serum MMP-9 and TIMP-1 were measured by ELISA in normal control group（n = 20）and 60 cases with CRF including azotemia （n = 15） ,renal failures（n = 24）and uremia（n = 21 ）, and general clinical data were collected,meanwhile. We analyzed the possible correlation between serum MMP-9,TIMP-1, ratio of MMP-9/TIMP-1 and clini- cal indexes of renal damage. Results The levels of serum MMP-9 and TIMP-1 were obviously elevated in CRF groups,while the ratio of MMP-9/TIMP-1 were markedly decreased. With the estimated decreasing glomerular filtration rate （GFR）, serum MMP-9 and TIMP-1 levels tended to decline, and the ratio of MMP-9/TIMP-1 inclined to increase. The levels of serum TIMP-1 and the ratio of MMP-9/ TIMP-1 had a high correlation with serum creatinine.GFR and hemoglobin levels. Conclusions serum MMP-9 and TIMP-1 were involved in the progression of CRF. The levels of serum TIMP-1 or the ratio of MMP-9/TIMP-1 could preferably reflect the severity of renal fibrosis.
出处
《临床肾脏病杂志》
2008年第1期12-14,共3页
Journal Of Clinical Nephrology
