摘要
目的筛选组织工程血管支架材料的最适孔径。方法用生物可降解材料聚β羟基丁酯（PHB）作为支架材料，运用盐析方法制成实心和不同孔径的膜片；将培养的第3代血管平滑肌细胞种植于膜片上，于培养的第1、3、7、11、14天，采用倒置显微镜下观察、苏木素-伊红（HE）染色、甲苯胺蓝染色、扫描电镜检查及噻唑蓝（MTT）比色法检测查明材料上细胞附着和生长情况。结果倒置显微镜下无法观察附着于材料上的细胞；HE和扫描电镜标本制备过程中细胞丢失多，其不能为材料提供准确的信息；甲苯胺蓝染色可快速观察材料上细胞附着情况；MTT比色法可定量测定材料上的细胞数。经比较发现150—200μm孔径的PHB膜片上血管平滑肌细胞附着和生长最佳。结论150—200μm孔径的PHB膜片最适合血管平滑肌细胞的附着和生长。
Objective To explore optimal pore caliber of scaffolds for tissue engineering vessel. Methods Scaffolds of various pore calibers were made by salt-eluting method using biodegradable poly-β- hydroxybutyrate （ PHB ）. Vascular smooth muscle cells were implanted in scaffolds of various pore calibers. Scaffolds were examined bu phase-contrast microscopy, HE staining, toluidine blue staining, scanning electronic microscope and MTT method on culturing days of 1,3,7,11,14 for finding how cells grew. Resuits Scaffolds of various pore calibers were successfully made. Cells in scaffolds couldnt be seen under phase-contrast microscopy, HE staining and scanning electronic microscope couldnt provide accurate information due to cells loss during specimen preparation. Toluidine blue staining was a rapid method to observe cells in scaffolds, and MTT method could quantitatively measure cells number in scaffolds. Scaffolds with pore caliber of 150-200 μm had the most cells. Conclusion Scaffolds with pore caliber of 150-200 μm is the most suitable for tissue engineering vessel.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第2期161-162,共2页
Chinese Journal of Experimental Surgery
关键词
血管
组织工程
支架
Vascular
Tissue engineering
Scaffold
