摘要
目的:观察蛙皮素对梗阻性黄疸大鼠肠黏膜屏障功能损害的保护作用。方法:结扎胆总管制备梗阻性黄疸大鼠模型后,皮下注射蛙皮素30μg.kg-1.d-1,tid,给药10 d。假手术组和黄疸模型组给予生理氯化钠溶液。末次给药8 h后取腹腔灌洗液作细菌定量培养,检测静脉血内毒素水平和血清谷丙转氨酶(ALT),取静脉血和肠系膜淋巴结进行细菌定性培养,同时测定末端回肠黏膜厚度和绒毛高度。结果:阻塞性黄疸组血清总胆红素较假手术组明显增高(P<0.05)。与阻塞性黄疸组比较,蛙皮素组静脉血内毒素明显降低[(129.58±13.79)vs(239.03±32.0)ng.L-1,P<0.05],谷丙转氨酶明显降低(P<0.05),静脉血和肠系膜淋巴结细菌培养阳性率也明显降低(P<0.05),腹腔灌洗液菌落计数明显降低[(113.7±8.0)vs(363.2±15.1)CFU.mL-1,P<0.05],回肠末端黏膜厚度和绒毛高度明显增加(P<0.05)。结论:蛙皮素可保护梗阻性黄疸时小肠黏膜屏障功能,减少肠道细菌易位和内毒素血症。
Objective：To investigate the protective effect of bombesin （BBS） on impaired intestine mucosal barrier in rats with obstructive jaundice. Methods： After the obstructive jaundice was induced by ligation of common bile duct, the rats were treated with subcutaneous BBS 30μg·（kg·d）^-1 or saline, tid, for 10 days. At 8 h after the end of treatments, the bacteria in the intra-abdominal fluid were quantitatively evaluated, the endotoxin level in blood was measured, the total bilirubin and ALT were determined, and the bacteria in the blood and mesenteric lymph nodes was cultured. Meanwhile, terminal ileum mucosa was observed under the electron microscope. Results ： Total bilirubin was significantly increased after bile duct ligation （P 〈 0.05）. BBS significantly decreased the level of endotoxin [ from （239.03 ± 32.0） ng· L^-1 to （129.58 ±13.79） ng·L^-1, P 〈 0.05 ], the activity of ALT （P 〈 0.05） , the positive rates of bacterial culture in the blood and the mesenteric lymph nodes （ P 〈 0.05 ）, and the quantitative cultures of intra-abdominal fluid [ from （ 363.2 ±15. 1 ） CFU· mL^-1 to （ 113.7 ± 8.0） CFU· mL^-1 , P 〈 0.05 ] in rats with obstructive jaundice. In addition, BBS markedly increased the thickness of terminal ileac mucous membrane and the length of villi （ P 〈 0.05 ）. Conclusion ： BBS has a protective effect on the impaired mucosa barrier in rats with obstructive jaundice, and can decrease endotoxemia and bacteria translocation.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2008年第4期292-295,共4页
Chinese Journal of New Drugs
基金
南京市科技局资助项目(200507003)
