摘要
新生一天的大鼠海马神经细胞在含10％胎牛血清和10％小牛血清的DMEM培养液,5％CO_2,37℃的环境中可以获得良好的生长。用从微摩尔浓度至毫摩尔浓度的铝处理细胞1小时,细胞死亡率明显增加,并呈剂量依赖性。但10^(-3)mol/L浓度的铝却使死亡率突然降低,与10^(-4)mol/L比较具有极显著的统计学意义。铝的神经毒作用与细胞外液中钙离子存在与否无关,不能用传统的兴奋性学说来解释。
Hippocampal neurons dissected from the one-day newly-born rats can be cultured well in DMEM medium supplemented with 10% fetal calf serum and 10% new-born bovine serum in a 37℃, 5%CO2 incubator. Cell mortality were remarkably increased in a dose-depentent man-ner, when the cell treated for one hour with aluminium of 10-6mol/L, 10-5mol/L, 10-4mol/L concentration respectively. But aluminium of 10-3mol/L caused a significant decline of cell mortality comparing with that of 10-4.urol/L Aluminium neurotoxicity has no relation with the extracellular calcium, which unable to be explained by the traditional excititoxity hypothesis.
出处
《细胞生物学杂志》
CSCD
1997年第3期136-139,共4页
Chinese Journal of Cell Biology
关键词
铝
毒性
海马
神经细胞
原代培养
神经毒性
Aluminium Neurotoxicity Hippocampal Neuron Primary culture
