摘要
目的研究HER-2癌基因反义寡脱氧核苷酸联合叶酸对乳腺癌细胞SK-Br-3化疗敏感性的影响。方法通过叶酸受体将HER-2反义寡脱氧核苷酸导入乳腺癌细胞,给予不同剂量的阿霉素和顺铂,用四氮唑盐(MTT)方法检测细胞存活率。结果反义寡脱氧核苷酸-叶酸(ASODN-FA)显著增加SK-Br-3细胞对阿霉素和顺铂的敏感性,使细胞存活率明显降低。结论HER-2反义寡脱氧核苷酸能增加乳腺癌细胞对阿霉素和顺铂的化疗敏感性。
Objective To study the effects of HER-2 oneogene antisense oligodeoxynudeotide-folate ASODN-FA HER-2 ASODNs , as a oncogene inhibitor, on chemotherapeutic drug sensitivity in human breast cancer cells line SK-Br-3. Methods Human breast cancer cells were cultured and inoculated into the wells, then added ASODN-FA 100 μl. The chemotherapeutic drug adriamycin（ADM） and cisplatin （DDP） were administrated different concentration to observe the effect of ASODN on the cell survival rate by MTT. Results ASON-FA HER-2 ASODNs inhibited the expression of HER-2 onco gene which significantly increased the chemosensitivity of breast cancer cells SK-Br-3 on ADM and DDP. Conclusion HER-2 antisense oligodeoxynucleotide can effectively enhance the sensitivity of human breast carcinoma cells to the chemotherapeuties drug adfiamycin and cisplatin.
出处
《贵州医药》
CAS
2008年第1期1-4,共4页
Guizhou Medical Journal
基金
贵州省优秀科技教育人才省长专项资金资助项目[黔省专合字(2005)36号]
关键词
HER-2原癌基因
反义寡脱氧核苷酸
乳腺癌细胞
化疗敏感性
HER-2 protooneogene Antisense oligodeoxynudeotides Human breast carcinoma cells Chemotherapeutic sensitivity
