摘要
采用MTT法研究蜂胶的超临界CO2萃取物（SE-P）对体外培养的肿瘤细胞生长的抑制作用,并和市售蜂胶乙醇提取物（ME-P）、蜂胶超临界CO2萃余物的95%乙醇提取物（RE-P）的抗肿瘤活性进行了对比;研究了不同萃取压力所得SE-P的抗肿瘤功效.SE-P在体外对肿瘤细胞U937,95D,SGC-7901和TE-1具有较强的抑制作用,SE-P对4株肿瘤细胞的最高抑制率和最高抑制率质量浓度均高于ME-P和RE-P.SE-P的抗肿瘤成分在各压力段都有分布,以10-15 MPa和20-30 MPa压力段抗肿瘤效果最好.研究结果表明,SE-P对体外培养的肿瘤细胞有较强的生长抑制作用.
In vitro anti-tumor activity of supercritical CO2 extract of propolis （SE-P） was studied by means of MTT assay and was compared with ethanol extract of propolis from market （ME-P） and 95% ethanol extract of propolis residue after being extracted by supercritical CO2 （RE-P）. Anti-tumor activity of SE-P gained under various extraction pressure was studied. SE-P has an obvious inhibitive effect on U937, 95D, SGC-7901 and TE-1 cell lines. Comparing to ME-P and RE-P, SE-P has the highest anti- tumor activity to above cell lines. The anti-tumor activity factors can be extracted within a wide pressure range. The best ranges are 10 - 15 MPa and 20 - 30 MPa. It shows that the SE-P has obvious anti-tumor activity on tumor cell lines.
出处
《江苏大学学报（自然科学版）》
EI
CAS
北大核心
2008年第1期5-8,共4页
Journal of Jiangsu University：Natural Science Edition
基金
江苏高校高新技术产业化项目(JH01-059)
