摘要
目的:观察急性心肌梗死大鼠心肌细胞凋亡率、凋亡调控基因Fas/FasL的表达变化及罗格列酮的干预作用。方法:通过结扎雄性Wistar大鼠左冠状动脉前降支造成心肌梗死模型,心肌梗死后24 h随机分为心肌梗死组(A组,n=18)和罗格列酮干预组(B组,n=18),另设假手术组(C组,n=18)。B组每日给以罗格列酮灌胃(4 mg/kg),持续6周。A、C组每日给以等量生理盐水灌胃。6周后检测心肌非梗死区中细胞凋亡率、细胞凋亡基因Fas mRNA和Fas/FasL蛋白的表达量。结果:给药6周后急性心肌梗死组A、B组心肌细胞中心肌细胞凋亡指数、Fas/FasL及其蛋白表达与C组相比显著升高,差异有统计学意义(P<0.05);与A组相比,B组中心肌细胞凋亡指数,Fas/FasL基因及其蛋白表达显著下降,差异有统计学意义(P<0.05)。结论:大鼠心肌梗死后心肌细胞中细胞凋亡率、Fas/FasL基因及蛋白表达显著增高;而罗格列酮可以降低大鼠心肌梗死后心肌细胞中Fas/FasL基因及蛋白表达,减少心肌细胞凋亡。
Objective To study the effect of rosiglitazone on myocyte apoptosis after myocardial infarction in rats. Methods After the model of myocardial infarction was established by ligating the left anterior descending coronary of rats, the survivals were randomly divided into two groups： group A （myocardial infarction group, n= 18） and group B（Rosiglitazone group, n= 18）. Meanwhile, the other 18 shame operation rats were as control group（group C, n= 18）. Each group was treated by direct gastric gavage from the second day, and the rats of group B were treated with rosiglitazone, group A and C with drinking water. Six weeks later, the myocyte apoptosis index was detected through TUNEL and the expression of Fas mRNA through SYBR Green I fluorescent quantitative PCR. And the protein of Fas/FasL was detected through ABC immunohistochemical staining. Results Compared with group C, there was a significant increase of apoptosis index（P〈0.05）. The apoptosis index, the level of the protein of Fas/FasL and the mRNA expression of Fas in group A were higher than group B （P 〈 0. 05 ）. Conclusion After myocardial infarction, the apoptosis index and the expression of Fas mRNA in myocardium are increased distinctly. Rosiglitazone, which can reduce the expression of Fas /FasL mRNA can lessen the degree of cell apoptosis.
出处
《实用诊断与治疗杂志》
2008年第3期183-185,共3页
Journal of Practical Diagnosis and Therapy
