摘要
采用稻壳作为基质,利用裂褶菌F17固态发酵产锰过氧化物酶(MnP),通过正交实验对发酵条件进行优化,并对5种不同结构类型的染料进行脱色.结果显示,含水率、温度、Mn2+、Cu2+对裂褶菌F17产MnP有显著影响;MnP发酵的最优化条件为稻壳27g、黄豆粉3g、MnSO40.4mg.g-1、含水率150%、接种量50%、培养温度22℃,pH不调节,优化后酶活达到16.39U.g-1,比优化前的酶活9.20U.g-1提高了78.2%.优化后的发酵体系对染料刚果红、茜素红、PolyR-478、中性红和结晶紫的24h脱色率分别达到92.6%、90.3%、93.1%、87.3%和95.6%.
Schizophyllum sp. F17 can use rice hulls to produce manganese peroxidase （MnP） by solid-state fermentation. Important parameters were optimized through orthogonal tests and five dyes with different structures were decolorized by applying MnP. The results showed that the moisture ratio, temperature, and concentrations of Mn^2＋ and Cu^2＋ all significantly influenced MnP production. The optimal conditions were rice hull 27 g, bean powder 3 g, MnSO4 0.4 mg·g^-1, moisture ratio 150%, inoculation amount 50%, culture temperature 22℃ and pH not adjusted. Under these conditions, the MnP activity was increased by 78.2% from 9.20 U·g^-1 to 16.39 U·g ^-1 and after 24 h of decolorization, the decolorization rates were 92.6% of Congo red, 90.3% of Alizarin red, 93.1% of Poly R-478, 87.3% of Neutral red and 95.6% of Crystal violet.
出处
《环境科学学报》
CAS
CSCD
北大核心
2008年第3期490-495,共6页
Acta Scientiae Circumstantiae
基金
安徽省自然科学基金研究项目(No.070413132)
安徽大学211工程学术创新团队基金(No.02203109)
安徽大学人才队伍建设项目~~
关键词
裂褶菌F17
固态发酵
锰过氧化物酶
稻壳
优化
染料脱色
Schizophyllum sp. F17
solid-state fermentation
MnP
rice hull
optimization
dye decolorization
