激光扫描共聚焦显微镜的原理及其在医学研究中的应用
被引量:1
摘要
随着科学技术的发展,实验技术已经成为实现实验目的的重要手段,对实验新技术的了解和掌握是科技工作者应具备的技能。激光扫描共聚焦显微镜(Laser Scan-ning Confocal Microscope,LSCM)是当今世界上最先进的分子细胞生物学分析仪器,利用激光、电子摄像和计算机图像处理系统,使用紫外光或可见光激发荧光探针,从而得到细胞或组织内部微细结构的荧光图像,
出处
《承德医学院学报》
2008年第1期83-85,共3页
Journal of Chengde Medical University
参考文献10
-
1Alexandmtou E,Yova D,Handris P, et al. Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy[J].Photochem Photobiol Sci,2002,1 (8):547-552.
-
2何其华.激光扫描共焦显微镜在检测活体组织和细胞中的应用[J].中国医学装备,2004,1(4):43-47. 被引量:9
-
3BeckA,Lohr C,Berthold H,et al.Calcium influm into dendrites of the leech Retzius neuron evoked by 5-hydroxytryptamine [J].Cell Calcium,2002,31(3): 137-149.
-
4Chan WY, Huang H,Tam SC. Receptor-mediated endocytosis of trichosanthin in choriocarcinoma cells[J].Toxicology, 2003386(3): 191-203.
-
5李福平,岳焕勋,徐克惠,蒋敏,林丽,乔林,张思孝.精子核成熟度与精液参数之间的关系[J].中华男科学杂志,2007,13(2):179-180. 被引量:10
-
6杨秋霞,郗雪艳,李波,李殿俊,吕雪莹,刘云鹏.单纯疱疹病毒1型诱导的Hela细胞凋亡及钙浓度的变化[J].中国免疫学杂志,2002,18(2):88-88. 被引量:5
-
7Chertin B,Rolle U,Farkas A,et al.The role of nitric oxide in reflux nephropathy [J].Pediatr Surg Int,2002,18(7):630-634.
-
8Cope SJ,Hibberd S,Whetstone J,et al.Measurement and mapping of pH in hydrating pharmaceutical pellets using confocallaser scanning microscopy[J].PharmRes,2002,19(10): 1554- 1563.
-
9Guan Y, Dong RWan C,et al.The assessment of brain injury with measurement of calcium fluorescent intensity of a vital brain slice[J].J ExtraCorporTechnol,2002,34(3): 185-189.
-
10Dimitrova M,Imbert I,Kieny MP, et al.Protein-protein interactions between hepatitis C vires nonstructural proteins[J].J Viral,2003,77(9):5401-5414.
二级参考文献11
-
1张平,张洪亚,王苏建,陈仰之.精液白细胞对精子功能的影响[J].中华男科学杂志,2005,11(6):466-467. 被引量:2
-
2[1]Leppardi R,Roizaman B.The Herpes simples virus major regulatory prote in ICP 4 block apoptosis induced by the virus or by hyperthermia[J].Pro Nat Ac ad Sci Vsa,1996;93:9583
-
3[2]Baffy G,Miyashita T,Williamson J R et al.Apoptosis induced by withdrawal of interleukin-3(IL-3) from and IL-3-dependent hematopoietic cell line is as sociated with repartitioning of intracellular calcium and is blocked by enforced Bcl-2 oncoprotein production[J].J Biol Chem,1993;268:6511
-
4[3]Reyhaneh F Y,Arnaudeau S,Borner C et al.Bcl-2 decreases the free Ca 2+ concentration within the endoplasmic reticulum[J].Proc Natl Acad Sci USA, 2000;97(11):5723
-
5[4]Lennon S V,Kilfeather S A,Hallett M B et al.Elevations in cytosolic free Ca2+ are not required to trigger apoptosis in human leukaemia cells[J].C lin Exp Immunol,1992;87(3):465
-
6[5]Oshimi Y,Miyazaki S.Fas antigen-mediated DNA fragmentation and apoptotic mor phologic changes are regulated by elevated cytosolic Ca2+ level[J].J Imm unology,1995;154(2):599
-
7Tejada RI, Mitchell JC, Norman A, et al. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence[J]. Fertil Steril, 1984, 44(1) : 87-91.
-
8Katayose H, Yanagida K, Hashimoto S, et a/. Use of diamideacridine orange fluorescence staining to detect aberrant protamination of human-ejaculated sperm nuclei [ J ]. Fertil Steril, 2003,79( Suppl 1 ) :670-676.
-
9Soderlund B, Lundin K. Choosing fertilization method by analyzing sperm morphology or by performing swim-up preparation[ J].Aeta Obstet Gyneeol Seand, 2006, 85 (3) : 306-311.
-
10Hoshi K, Katayose H, Yanagida K, et al. The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm [ J ]. Fertil Steril, 1996, 66 (4) : 634 -639.
共引文献21
-
1刘继红,蔡学泳,何其华.细胞骨架的激光共焦研究技术[J].中国医学装备,2005,2(6):49-51. 被引量:8
-
2刘红云,唐省三,姜益泉.HSV-1心肌炎心肌凋亡细胞的检测与分析[J].中国现代医学杂志,2005,15(18):2770-2772. 被引量:4
-
3刘继红,汪成楚.激光扫瞄共聚焦显微技术实验教学的思考[J].实验技术与管理,2006,23(1):86-87. 被引量:11
-
4杜江平,李艳,杨书文,门金娥,张向阳,郑海萍,华咏.自拟中西药处方治疗少弱畸精子综合征疗效观察[J].辽宁中医杂志,2007,34(10):1445-1446.
-
5杜江平,杨书文,门金娥,王霞,张向阳,郑海萍,李艳.少弱畸精子综合征治疗前后精子染色质流式细胞术分析[J].中华男科学杂志,2008,14(4):334-337. 被引量:2
-
6沈丽萍,朱冬发.激光扫描共聚焦显微镜在海洋动物发育生物学上的应用[J].海洋湖沼通报,2007(B12):216-222. 被引量:1
-
7瞿文军,蔡颖谦,徐如祥.创伤性脑损伤活体脑片不同时相的脂质过氧化损伤[J].热带医学杂志,2008,8(7):660-663. 被引量:1
-
8王玉芝,张向宇,刘剑,杜彩瑞.柠檬提取物抗单纯疱疹病毒实验研究[J].实用口腔医学杂志,2008,24(5):627-629. 被引量:7
-
9张丽红,邱毅,王磊光,王苏梅.精子染色质扩散试验及吖啶橙染色试验检测精子DNA完整性研究[J].中华检验医学杂志,2008,31(12):1335-1339. 被引量:6
-
10刘居理,罗明,卢雪芳,杜俊.男性不育患者精子核蛋白组型转换与精子密度及活力分析[J].检验医学与临床,2009,6(13):1061-1062. 被引量:6
同被引文献11
-
1葛元输,石四箴,许世梃.幼儿唾液中的钙含量及其与患龋状况的关系[J].牙体牙髓牙周病学杂志,2004,14(6):306-308. 被引量:3
-
2Shimono T,Mjzuno J,Nonomura E,et al.Studies on a new caries activity test(cariostat):Comparison with the Snyder Test.Japan J Pedod,1976,14:6.
-
3Kittner D,Beetke E,Kotzschke R.Contents of phosphate and calciumin whole-saliva of test persons with high and low caries prevalence.Stomatol DDR,1990,40(5):217-219.
-
4Tunwell RE,Lai FA.Ryanodine receptor expression in the kidney and a nonexcitable kidney epithelial cell.J Biol Chem,1996,271(47):29583-29588.
-
5Berridge MJ.Inositol trisphosphate and calciumsignalling.Nature,1993,361(6410):315-325.
-
6Murty VL,Slomiany BL,Laszewicz W,et al.Lipids of developing dental plaque in caries-resistant and caries-susceptible adult people.Arch Oral Biol,1985,30(2):171-175.
-
7Hayes ML,Stobart HJ.The effects of mediumchain fatty acids and fluoride on fissure caries in Wistar rats.Arch Oral Biol,1990,35(12):939-943.
-
8周琼,白洁,秦满.致龋菌、唾液缓冲能力与婴幼儿龋的相关性研究[J].中华口腔医学杂志,2007,42(10):581-584. 被引量:7
-
9梁勤,许强,石四箴.幼儿腭粘膜细胞钙离子浓度和龋病关系的研究[J].现代口腔医学杂志,2008,22(4):365-367. 被引量:2
-
10吕宁舟,邬黎明.唾液PH值与龋病的关系研究[J].海南医学,2002,13(9):41-42. 被引量:8
-
1郎丽丽.数学建模在医学中的应用[J].科学中国人,2016(9Z).
-
2娄晋宁.微血管内皮细胞的培养及其在医学研究中的应用[J].微循环学杂志,2004,14(3):5-8. 被引量:26
-
3郭长占,田枫,王兆绰.转基因动物在医学研究中的应用[J].实验动物科学与管理,1999,16(2):42-44.
-
4王颖慧,刘红霞,丁振华.细胞外MicroRNA及其生物学作用[J].生理科学进展,2014,45(4):295-298.
-
5崔美芝,刘恩重,柳英侠.长爪沙鼠在医学研究中的应用[J].哈尔滨医科大学学报,1996,30(2):205-206. 被引量:9
-
6李卫党,范丽安,等.DNA芯片技术及其在医学研究中的应用[J].微生物学免疫学进展,2001,29(3):69-72. 被引量:2
-
7张玮,彭澎,沈铿.外泌体来源RNA在细胞通讯中的作用[J].中国医学科学院学报,2016,38(4):480-483. 被引量:13
-
8岳剑宁,孔繁元.共聚焦激光扫描显微镜技术及相关荧光探针在细胞器研究中的应用[J].中华病理学杂志,2002,31(3):266-267. 被引量:2
-
9提高临床疗效 实现个性化疗——病理科开展肿瘤药敏实验新技术[J].家庭健康(医学科普),2005(10):44-45.
-
10李瑞峰,魏树珍,陈融,郭成浩,陈福琴,李莉,邴鲁军.一种外周血白细胞原位杂交实验新技术[J].山东大学学报(医学版),2002,40(3):277-278.