摘要
目的通过对呼吸道标本真菌培养与涂片镜检结果的比较分析,评价其在下呼吸道真菌感染实验诊断中的应用价值。方法常规收集临床送检呼吸道标本620例进行真菌培养及鉴定与涂片镜检,比较其结果并结合患者临床资料进行分析。结果620例呼吸道标本中,真菌培养阳性201例,阳性率为32.4%,其中白色念珠菌164例,占培养阳性的81.6%,其次是光滑念珠菌及热带念珠菌;曲霉菌4例,占培养阳性的2.2%。涂片镜检阳性133例,其中,查见真菌孢子及菌丝81例,占镜检阳性的60.9%,查见真菌孢子未见菌丝49例,占镜检阳性的36.8%,仅查见菌丝3例。结论真菌培养及鉴定有助于确定其种类,但耗时较长,且容易受到上呼吸道定植真菌的干扰。真菌涂片镜检操作简便、快速,尤其是查见大量孢子及菌丝时对临床快速确定下呼吸道真菌感染具有重要意义,提示临床送检呼吸道标本作真菌培养及鉴定时应同时作真菌涂片镜检。
Objective To compare and analyze the results of sputum fungus culture and smear test, and so as to evaluate their applicated value in the laboratory diagnosis of lower respiratory infection. Methods 620 cases of sputum samples were collected for fungus culture and smear test, the detected results and the clinical data were analyzed. Results In 620 cases of sputum samples,201 cases （32.4%） were fungus culture positive, including Candida albicans （164/201, 81.6 %）, Candida glabrata, Candida tropicalis and Aspergilli （4/201, 2.2%）, and 133 cases were smear test positive, in which 81 cases（60.9 %） were fungal spore and mycelial double positive, 49 cases（36.8%） were fungal spore positive and mycelial negative 3 cases were only mycelial positive. Conclusion Fungus culture and identification is helpful for ascertainment of its category, but susceptible to interference of fungi field planting in the upper respiratory tract. By comparison, smear test is convenient and rapid, especially occurrence of a great quantity of fungal spore and mycelial contributing to definition of fungus infection in the lower respiratory tract. The results show that fungus smear test should be performed simultaneously while fungus culture and identification are carried out for clinical species from the respiratory tract.
出处
《国际检验医学杂志》
CAS
2008年第2期124-126,共3页
International Journal of Laboratory Medicine
关键词
真菌
呼吸道感染
实验室技术和方法
Fungi Respiratory tract infections
Laboratory techniques and procedures
