摘要
【目的】了解各个发育时期控制水稻分蘖数QTL的"静态"和"动态"信息。【方法】利用单片段代换系对不同时期的水稻分蘖数QTL同时进行非条件和条件定位分析。【结果】(1)水稻分蘖数至少受14个QTL影响,它们分布在第1、2、3、4、6、7和8号共7条染色体的相应代换片段上;(2)各个时期影响水稻分蘖数的QTL数量(变动在6～9之间)和效应(变动在1.49～3.49之间)均不相同;(3)水稻分蘖数QTL的表达具有很强的时序性,主要集中在移栽后0～7d(有6个正表达),14～21d(有9个随机表达)和35～42d(有6个负表达)3个时间段内,正、负表达分别决定了最高分蘖和有效分蘖的数量;(4)每个QTL在整个生育期至少表达1次,有些可多次表达;(5)某个时期的分蘖数量取决于所有QTL的累积效应,而某个时间段内分蘖数的增减量则取决于所有QTL的净效应。【结论】用单片段代换系和条件QTL定位方法对发育性状进行QTL定位分析非常有效和准确。
【Objective】 Research was carried out to understand the static and dynamic information of the QTLs for tillers in rice.【Method】Unconditional and conditional QTL were simultaneously mapped by using single segment substitution lines for tiller number at various stages in rice.【Results】（1） At least 14 QTLs influenced tiller number in rice, distributing on the corresponding substitution segments of seven chromosomes with the number of 1, 2, 3, 4, 6, 7 and 8, respectively. （2） Both the number and the effect of tiller number QTLs were different at various stages, varying from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. （3） Tiller number QTLs expressed timely, mainly arising at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with six positive, nine random and six negative expressing QTLs, respectively, which determined the number of the up most and the effective tillers in sequence. （4） Each of the QTLs detected expressed at least one time or more during the whole duration of rice growth. （5） The tiller number was determined by sum of QTL effects estimated by unconditional method, while the number of increasing or decreasing was controlled by total of QTL effects estimated by conditional method at a given stage.【Conclusion】 It is highly effective and accurate to map QTLs by using both materials of single segment substitution lines and conditional analysis methodology.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第2期322-330,共9页
Scientia Agricultura Sinica
基金
国家自然科学基金重点项目(30330370)
关键词
单片段代换系
非条件QTL定位
条件QTL定位
分蘖数
水稻
Single segment substitution lines
Unconditional QTL mapping
Conditional QTL mapping
Tiller number
Rice
