摘要
目的:建立一种简便快速的检测囊虫抗体的斑点免疫金渗滤法(DIGFA)。方法:将毕赤酵母重组表达的猪囊尾蚴抗原cC1点于硝酸纤维素膜上,用来检测血清中囊虫抗体,通过胶体金标记的金黄色葡萄球菌A蛋白直接显色,阳性者出现红色斑点,结果易于判断。结果:DIGFA检测40份囊虫病患者血清和40份正常人血清,敏感性为95%(38/40),特异性为100%(40/40)。检测20份包虫病患者血清,3例阳性,交叉反应率为15%(3/20);检测20份肝吸虫病患者血清,20份肺吸虫病患者血清,20份血吸虫病患者血清,均未出现交叉反应。与酶联免疫吸附试验对比,检测的总符合率为95%,差异无显著性(P﹥0.05)。结论:DIGFA简便、快速、准确,适于现场应用,可用于囊虫病的诊断及流行病学调查。
Objective To establish a new simple rapid dot immunogold filtration assay （DIGFA） for detecting antibody of cysticercus cellulosae in patient serum. Methods DIGFA was developed by coating the common antigen 1 of cysticercus cellulosae on nitrocellulose membrane, labeling staphylococcal protein A with colloidal gold. The color of the positive dots were so bright that the results could be easily determined. Results Sensitivity and specificity of DIGFA were 95% （38/40） and 100% （40/40）, respectively. Fifteen percent of cross reaction was with sera of echinococcosis patients. No cross reaction was showed with other helminthiasis such as clonorchiasis, paragonimiasis and schistosomiasis. The coincidence rate of the results of simultaneous determination of serum with enzyme-linked immunosorbent assay and DIGFA was 95% for antibody of cysticercus. Conclusions DIGFA , more simple more rapid and accurate, is applicable to field investigation. It can be applied for diagnosis and epidemiological investigation on cysticercosis.
出处
《实用医学杂志》
CAS
2008年第4期652-653,共2页
The Journal of Practical Medicine
关键词
囊虫病
抗体
斑点免疫金渗滤法
Cysticercosis Antibodies Dot immunogold filtration assay
