摘要
建立了石墨炉原子吸收光谱法测定蚕蛹中Cr、Se的方法,为蚕蛹新资源食品的开发及明确其营养价值提供科学数据。利用石墨炉原子吸收光谱法,偏振塞曼效应扣除背景,石墨炉程序升温方式进行Cr、Se的原子化,检测峰值吸收。加入硝酸镍、吐温X-100为基体改进剂,在体积分数0.5%HNO3介质中对桑蚕蛹中Cr、Se进行测定。方法的精密度：4.0%（Cr）,3.1%（Se）。加标回收率：96.8%-105.3%（Cr）,92.1%-108.8%（Se）。Cr、Se的线性范围和检出限分别为：0-10μg/L（Cr）,0-40μg/L（Se）;LOD=1.22μg/L（Cr）,1.86μg/L（Se）。建立的分析方法适用于蚕蛹中Cr、Se的测定。
A method was developed for the determination of chromium and selenium in silkworm pupa using electrothermal atomic absorption spectrometry. The use of chemical modifiers as well as their implications during the pyrolysis step was examined. The chemical modifiers that were studied were Ni, Pd, Mg. The effect of modifiers was studied and it was found that better results were achieved with addition of 1 mg/mL Ni（NO3 ）2. The developed method was applied to the determination of chromium and selenium in silkworm pupa, which was digested with a HNO3 ＋ HClO4 in a triangle bottle. The repeatability of the method was less than 4.0% as RSD（ % ）. Recoveries were estimated to be 96.8% - 105.3% for Cr, 92.1% - 108.8% for Se. The linear ranges of Cr and Se were 0- 10.0 μg/L, 0-40.0 μg/L, respectively. The limit of detection for Cr was 1.22 μg/L, The limit of detection for Se was 1.86 μg/L. This method is simple, accurate and highly specific. The method was applied to silkworm pupa and the Cr, Se contents were found to be 1.821 ± 0.073 ng/g, 0.458 ±0.014 ng/g, respectively. This method can be used for the determination of Cr, Se in usual food.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第3期38-41,共4页
Chinese Journal of Analysis Laboratory
基金
河南省教育厅自然科学基金(200510459066)项目资助
关键词
蚕蛹
CR
SE
石墨炉原子吸收光谱法
测定
Silkworm pupa
Chromium
Selenium
Electrothermal atomic absorption spectrometry
Determination
