摘要
目的分析多个患者多种标本中分离的耐碳青霉烯鲍曼小动杆菌的传播及分子特征。方法用WHONET5．4软件分析耐碳青霉烯鲍曼不动杆菌菌株的一般情况，结合患者病历复习，了解所分离菌株的传播和致病作用；利用脉冲场凝胶电泳（PFGE）分析基因型，采用多重PCR检测碳青霉烯酶基囚，确定菌株的分子流行特征以及相白：之间的亲缘关系。结果从13例患者的2种或2种以上来源的标本中共狭得耐碳青霉烯鲍曼小动杆菌29株。PFGE分型显示共有两种不同基因型，其中A型22株，来自11名患者；B型7株，来自4名患者。PCR扩增结果显示，除1株菌外，所有菌株均含有碳青霉烯酶基闲OXA23；除3株菌外，均含有Ⅰ类整合子基冈。结论所检测的耐碳青霉烯的鲍曼不动杆菌有2个基因型，以A型为主。绝大多数鲍曼不动杆菌含OXA-23型碳青霉烯酶基因和Ⅰ类整合子基因。该菌很容易通过患者自身传播及相互传播，引起医院内耐药菌感染流行。
Objective To study the mode of transmission and molecular characteristics on carbapenem-resistant Acinetobacter baumannii strain. Strains were isolated from different parts of samples in various patients. Methods Clinical information of earbapenem-resistant Acinetobacter baumannii isolates were stored and analyzed by WHONET 5.4 software. The transmission and pathopoiesis of the strains were learned through ease file review. Genotypes of isolates were identified by pulse-field gel electrophoresis （PFGE） and genes of earbapenemase were detected by multiple PCR, in order to find molecular characteristics and relatedness between strains. Results 29 stains of Acinetobacter baumannii resistant to earbapenem were isolated from 2 or more kinds of samples among 13 patients . Two genotypes were identified by PFGE： genotype A was obtained from 22 isolates in 11 patients and genotype B was obtained from 7 isolates in 4 patients. PCR amplification showed that all strains possessed OXA-23 gene except 1 ,and all strains possessed Integrasegene I except 3. Conclusion There were 2 different genotypes from 29 strains of carbapenm-resistant Acinetobacter baumannii with Genotype A as the main type. OXA- 23 carbapenmase gene and integrase gene Ⅰ were detected from most of the isolates. All the strains could be easily transmitted in the body of the patients and among patients, hence beeaming the epidemic pathogen of iatrogenie infection.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2008年第3期277-281,共5页
Chinese Journal of Epidemiology
基金
基金项目：军队“十一五”课题资助项目（06MA294）
关键词
鲍曼不动杆菌
碳青霉烯类
医院感染
Acinetobacter baumannii
Carbapenem
Nosocomial infections
