摘要
目的：利用HPLC法同时测定苹果中9种多酚物质的含量；方法：色谱柱为C18柱（150mm×4．6mm，5μm），以乙腈和醋酸-醋酸钠缓冲溶液为流动相，采用不同的流速和浓度梯度洗脱的分离条件，并用该方法测定印度青苹果中多酚的含量；结果：中性酚和酸性酚在该条件下得到很好的分离，分离度均大于1．5，该方法的加标回收率均在90％以上，相对标准偏差为1．2％～2．3％。结论：该方法操作条件简单，结果准确可靠。
Objective： Contents of nine kinds of polyphenol were determined by HPLC; Method： The neutral phenol and the phenolic acids were separated in a C18 column（ 150 mm ×4.6 mm,5μm）. The mobile phase was acetonitrile / acetic acid - sodium acetate buffer on gradient elution at the different flow rate. Contents of polyphenol in the Yinduqing apple were determined by this method;Result： The resolution is all above 1.5 and the recovery rate exceeds 90%. The relative standard deviation is 1.2% - 2.3% ; Conclusion： This method is simple, accurate and reliable.
出处
《食品与机械》
CSCD
北大核心
2008年第1期127-129,151,共4页
Food and Machinery
基金
烟台大学青年基金资助(项目编号:HY06Z11)
关键词
HPLC
多酚
苹果
High Performance Liquid Chromatography
Polyphenol
Apple
