摘要
目的探讨糖皮质激素治疗对系统性红斑狼疮（SLE）患者外周血CD4^＋ Foxp3^＋T细胞水平的影响以及CD4^＋ Foxp3^＋T细胞与SLE疾病活动的相关性。方法采用流式细胞术检测26例SLE患者和5名正常人外周血CD4^＋ Foxp3^＋T细胞百分率，Spearman相关分析法分析CD4^＋ Foxp3^＋T细胞与SLE疾病活动指标及糖皮质激素的相关性。结果活动期SLE患者外周血CD4^＋ Foxp3^＋T细胞百分率（2.4±1.6）％低于正常人（3.3±0.8）％，但差异无统计学意义；非活动期SLE患者外周血CD4^＋ Foxp3^＋T细胞百分率（3.3±0.7）％与正常人比较差异无统计学意义。活动期SLE患者经糖皮质激素治疗后外周血CD4^＋ Foxp3^＋T细胞（6.1±3.5）％较治疗前（3.9±2.4）％升高（P〈0.05）。SLE患者外周血CD4^＋ Foxp3^＋T细胞水平与年龄、病程、红细胞沉降率、尿蛋白定量（24h）、血清补体c3浓度、血清抗双链DNA（anti-dsDNA）抗体水平及SLE疾病活动指数（SLEDAI）无相关性，但与糖皮质激素每日用量呈显著正相关（r=0．51，P〈0．05）。结论SLE患者外周血CD4^＋ Foxp3^＋T细胞水平不能作为狼疮活动指标。糖皮质激素治疗可上调SLE患者外周血CD4^＋ Foxp3^＋T细胞水平，CD4^＋ Foxp3^＋T细胞水平上调可能是糖皮质激素治疗SLE有效的机制之一。
Objective To investigate whether the number of CD4^＋ Foxp3^＋ T cells is abnormal in patients with systemic lupus erythematosus （SLE） and observe the regulation of glucocorticoid on CD4^＋ Foxp3^＋ T cells in SLE. Methods The percentage of CD4^＋ Foxp3^＋ T cells in peripheral blood was detected by flow cytometry of 26 SLE patients and 5 healthy controls. Linear regression was used to analyze correlation between the percentage of CD4^＋ Foxp3^＋ T cells and lupus activity. Results The percentage of CD4^＋ Foxp3^＋ T cells in active SLE patients （2.4±1.6）% was lower than that in both inactive SLE （3.3±0.7）% and normal controls （3.3±0.8）%, but no significant difference could be found. The percentage of CD4^＋ Foxp3^＋ T cells in SLE pa- tients treated with steroids was increased compared with those without treatment [（6.1±3.5）% vs （3.9±2.4）%, P〈0.05 ]. There were no significant correlations between the percentage of CD4^＋ Foxp3^＋ T cells and SLE disease active index （SLEDAI）, 24 hours urine protein, serum complement C3 concentration, serum levels of anti-dsDNA antibody, erythrocyte sedimentation rate （ESR）, age and disease duration. Whereas the percentage of CD4^＋ Foxp3^＋ T cells was positively correlated with the daily dosage of glucocorticoid （r=0.51, P〈0.05）. Conclusion No significant difference of the percentage of CD4^＋ Foxp3^＋ T cells can be found between SLE patients and normal controls. The level of CD4^＋ Foxp3^＋ T cells in blood could not be marker for lupus activity. Glucocorticoid treatment upregulates the expression of CD4^＋ Foxp3^＋ on T cells in patients with SLE.
出处
《中华风湿病学杂志》
CAS
CSCD
2008年第3期166-169,共4页
Chinese Journal of Rheumatology
基金
基金项目：教育部高等学校博士点专项基金资助项目（20050315001）
江苏省自然科学基金资助项目（BK2006007）
江苏省135重点人才培养基金资助项目（RC2002003）
