摘要
目的:观察血小板衍生生长因子B链(PDGF-B)在大鼠根尖周炎中的表达情况,探讨PDGF-B与根尖周骨吸收的关系。方法:将24只SD大鼠开髓,分别于术后7,14,21,28 d取下颌骨,制备组织切片,用免疫组织化学的方法测定PDGF-B在大鼠根尖周炎中的表达,酶组织化学方法检测抗酒石酸酸性磷酸酶,观察破骨细胞。结果:在根尖周炎的急性期,即术后7 d和14 d,PDGF-B阳性细胞和破骨细胞都增多,二者存在明显正相关;在根尖周炎的慢性期,即术后21 d和28 d,PDGF-B表达继续增高,而破骨细胞数却迅速下降,二者为负相关。结论:PDGF-B在根尖周炎早期可能主要发挥促骨吸收作用,而在慢性期,PDGF-B可能主要与根尖周炎的修复相关。
Objective： To examine the presence of platelet-derived growth factor （PDGF）-B in periapical lesions in rats in order to discuss the relationship between PDGF-B and periapical bone resorption. Methods： Twenty-four SD rats were sacrificed at day 7, 14, 21 and 28 respectively after the mandibular first molar pulps were exposed. The jaws that contained the first molar were obtained and tissue slices were routinely prepared. Immunohistochemistry was used to detect the expression of PDGF-B in the periapical lesions of mandibular first molars of 24 rats, and enzymehistochemistry was used to detect the expression of TRAP in the periapical lesions. Results： From day 7 to day 14, the numbers of PDGF-B positive cells and osteoclasts were increasing, and a significant correlation was found between them. In the 214 and 28-day samples, more PDGF-B positive cells and fewer osteoclasts could be observed, and the positive expression of PDGF-B was negatively correlated with osteoclast of periapical lesions. In the earl resorption of periapical tissues, S. y b associated with the healing of the Conclusion： PDGF-B may be associated with the pathogenesis stage of periapical lesions, it may play leading roles in the bone ut in the later stage of periapical lesions, PDGF-B may mainly inflammation.
出处
《武汉大学学报（医学版）》
CAS
2008年第2期272-274,I0006,共4页
Medical Journal of Wuhan University
