摘要
目的研究Ｍｒ７２０００Ⅳ型胶原酶（ＭＭＰ＿２）在肝癌细胞中的表达与肝癌侵袭转移的关系．方法以原位分子杂交及免疫组化方法检测３４例原发性肝癌及其癌旁组织中ＭＭＰ＿２ｍＲＮＡ及其蛋白的表达．患者３０例伴肝硬变，４例无肝硬变，其中２４例肝癌突破包膜向周围肝组织侵袭性生长、门静脉癌栓形成或形成转移灶，１０例肝癌包膜完整．结果呈侵袭性生长的２４例肝癌中１６例癌细胞ＭＭＰ＿２ｍＲＮＡ表达明显增高，而包膜完整的１０例肝癌中仅有２例ＭＭＰ＿２ｍＲＮＡ表达阳性．侵袭性生长的肝癌２４例中１８例ＭＭＰ＿２呈不同程度的阳性表达，而包膜完整的肝癌１０例中仅３例ＭＭＰ＿２表达阳性，向外突出生长的肝癌细胞ＭＭＰ＿２表达阳性，转移组肝癌（发生门静脉癌栓或转移）ＭＭＰ＿２阳性率（８３３％，１５／１８）明显高于未转移组肝癌ＭＭＰ＿２阳性率（３７５％，６／１６）（Ｐ＜００１）．结论由肝癌细胞产生的ＭＭＰ＿２与肝癌的侵袭转移密切相关．
AIM To investigate the relationship between M r 72000 type Ⅳ collagenase (MMP_2) expression and hepatocellular carcinoma (HCC) invasion and metastasis. METHODS MMP_2 mRNA and its protein was tested in 34 HCC surgical specimens by using in situ hybridization and immunohistochemical techniques. There were 30 HCC in cirrhotic liver and 4 HCC in non_cirrhotic liver, 24 cases showed invasive growth or portal cancer emboli or metastasis, and 10 cases had intact surrounding membrane. RESULTS The MMP_2 expression in HCC cells was higher in 16 and 18 out of 24 invasive cases than that of their pericarcinomatous tissues in mRNA and its protein level, respectively. While in 10 non_invasive cases, only 3 showed MMP_2 positive expression in their HCC cells. The MMP_2 positive rate was higher in metastatic HCC (83 3%, 15/18) than that of non_metastatic HCC (37 5%, 6/16), and the most intensely positive areas were found near the periphery of the neoplasms. CONCLUSION The MMP_2 expression in HCC cells may be correlated with the invasion and metastasis of HCC.
关键词
肝癌
胶原酶
肿瘤转移
collagenases/metabolism
liver neoplasms/pathology
liver neoplasms/enzymology
neoplasm metastasis
