摘要
目的：建立HPLC法测定打碗花根中莨菪亭含量的方法。方法：采用Hypersil ODS2 C18色谱柱（4．6mm×250mm，5μm），流动相为乙腈1％冰醋酸水溶液（20：80），检测波长为341nm。结果：在1．07～21．4mg·L^-1范围内莨菪亭呈现良好的线性关系（r=0．9999）；平均回收率为99．2％，RSD为1．06％。结论：用本法测定打碗花根中莨菪亭含量灵敏准确，为其质量评价提供了一定的参考依据。
OBJECTIVE To develop a HPLC method to determine the content of scopoletin in root of Calystegia hedracea Wall. METHODS The separation was accomplished on a Hypersil ODS2 C18 column（4. 6 mm × 250 mm,5 μm）with a mixture of acetonitrile - 1% aqueous acetic acid（20： 80）as the mobile phase. The detecting wavelength was 341 nm. RESULTS The linear range was 1.07- 21.4μg·mL^-1 （r= 0. 999 9） ;The average recovery of the assay was 99. 2%, RSD= 1.06%. CONCLUSION This method had adequate sensitivity and accuracy to measure the concents of scopoletin and could be used in the quality control in the root of calystegia hedrecea Wall.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2008年第5期366-368,共3页
Chinese Journal of Hospital Pharmacy
基金
山西省科技攻关项目(编号:2006031078)
