摘要
目的阐明重庆地区庚型肝炎病毒感染状况和序列特征。方法以公布的 HGV 非编码区部分序列为引物,采用RT-套式 PCR 技术检测 HGV RNA,扩增产物序列采用 PCR 片段直接循环测序法测定。结果从73例各种类型肝炎患者血清中检出23例 HGV RNA 阳性,阳性率高达37%,其中26例非 A-E 型肝炎中检出6例阳性(23%),39例乙型肝炎患者中检出15例阳性(38%),测序结果表明,重庆株 HGV 非编码区序列与日本株较为接近,部分区段达96%,但完整序列与已知序列比较差异较大,平均同源性只有82%,且存在不同程度的插入和缺失突变。结论以上结果提示重庆地区可能为 HGV 高度流行区,是否提示重庆地区存在不同亚型的 HGV 感染,有待进一步实验证实。
Objective To investigate the prevalence of hepatitis G virus infection in Chongqing area.Methods Two sets of nested primers located in noncoding region of HGV genome were used to detect HGV RNA by RT-PCR. Results In 73 cases of different hepatitis patients,24 cases were positive for HGV RNA(37%).The positive rates of HGV RNA were 23%(6/26)in patients with non A-E hepatitis and 38%(15/39)in patients with hepatitis B respectively.Conclusions These results indicated that HGV infection was popular in the population of Chongqing.The nucleotide sequence analysis of NCR PCR product showed there were some insertions and deletions in the non-coding region of HGV RNA compared with HGV genome published.The possibility of infection with different subtype of HGV is investigating.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第1期5-7,共3页
Chinese Journal of Hepatology
