摘要
目的研究苦参碱对人高转移卵巢癌细胞系HO-8910PM增殖及转移相关能力的影响及其作用机制。方法以MTT法和台盼蓝拒染法检测苦参碱对人高转移卵巢癌HO-8910PM细胞增殖的影响;Transwell小室法检测其对HO-8910PM细胞侵袭运动能力的影响;考马斯亮蓝染色法检测不同质量浓度苦参碱对HO-8910PM细胞细胞骨架的改变及免疫细胞化学方法检测药物作用前后HO-8910PM细胞Ezrin蛋白的表达。结果苦参碱对HO-8910PM细胞增殖抑制呈明显剂量和时间效应;1.5g/L苦参碱能明显抑制细胞的增殖,作用6h后能抑制HO-8910PM细胞体外侵袭人工基底膜和运动能力,抑制率分别为(41.52±10.76)%和(39.73±10.67)%;1.0、1.5g/L苦参碱作用HO-8910PM细胞24h后使细胞骨架明显改变,并上调Ezrin蛋白的表达。结论苦参碱能抑制高转移性人卵巢癌细胞HO-8910PM的增殖能力和侵袭运动能力,其抗肿瘤侵袭运动的机制与细胞骨架和Ezrin蛋白表达的改变有关。
Objective To study the effect of matrine on proliferation and metastasis associated the abilities of human highly metastatic ovarian cancer HO-8910PM cells and its possible mechanism. Methods The trypan blue staining and MTT assay were used to examine the effect of matrine on proliferation of HO-8910PM cells; Transwell chamber assay was performed to determine the effect of matrine on the inva- sive and migratory abilities of the cells; Coomassie brilliant blue staining assay was used to detect the changes in cytoskeleton treated by matrine at different concentrations and immunocytochemical methods were used to observe the expression of Ezrin protein in HO-8910PM cell line befores and after matrine treatment. Results There was an obvious dose-dependent and time-consuming effect on the inhibition of HO-8910PM cells proliferation by matrine; 1.5 g/L matrine inhibited cell proliferation significantly, for 6 h after the treatment in vitro, the invasive and migratory abilities of HO-8910PM cells were also obviously down-regulated with the inhibitory rates （41. 52-1-10. 76）% and （39. 73±10. 67）%, respectively; Matrine distinctly changed the cytoskeleton and up-regulated the expression of Ezrin protein in HO- 8910PM cell line treated with 1.0 g and 1.5 g/L matrine for 24 h. Conclusion Matrine inhibits the proliferation, migration, and invasion abilities of HO-8910PM cell line in vitro, which may associate with the changes of cytoskeleton and Ezrin protein expression.
出处
《中草药》
CAS
CSCD
北大核心
2008年第3期386-390,共5页
Chinese Traditional and Herbal Drugs
基金
广东医学院青年科研基金资助项目(XQ0205)
广东省湛江市科技立项课题(ZK0535)
关键词
苦参碱
卵巢癌
增殖
侵袭
细胞骨架
EZRIN
matrine
ovarian carcinoma
proliferation
invasion
cytoskeleton
Ezrin
