摘要
环孢菌素A高产菌株No.421502经形态学特征和分子标记鉴定,确认为镰孢菌属,命名为Fusariumsp.No.421502.收集Fusarium sp.No.421502孢子刚萌发的幼嫩菌丝体,以0.7 mol/L KCl为渗透压稳定剂,用质量浓度为20 mg/mL的蜗牛酶,30℃80 r/min处理5 h制备原生质体.原生质体为7×105/mL,原生质体形成率为62.8%.制备的原生质体稀释后接入再生培养基,再生率为29.6%.
One Cyclosporin a high-yielding strain No. 421502 was identified as Fusarium species and named as Fusarium sp. No. 421502 by morphological characteristics and molecular marker, Young mycelia, germinated from Fusarium sp. No. 421502 spores, was collected and disposed with 0. 7 mol/L KCI as osmotic buffer by 20 mg/mL of snailase at 30 ℃ 80 r/min for 5 h to prepare protoplasts. Protoplast concentration was 7×10^5/mL, protoplast preparation rate was 62.8%. The protoplast was diluted and inoculated onto regeneration media, given the regeneration rate 29. 6%.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第2期63-66,79,共5页
Journal of Fujian Normal University：Natural Science Edition
