摘要
目的探讨缺血缺氧对左心室流出道电活动的影响及炙甘草汤的抗心律失常作用。方法利用常规的玻璃微电极细胞内记录的方法,观察正常灌流液、缺血缺氧灌流液和缺血缺氧+炙甘草汤(40mg/mL)灌流液对豚鼠左心室流出道慢反应自律细胞动作电位时程(APD)、50%复极化时间(APD50)、90%复极化时间(APD90)、4相自动去极速度(VDD)及自发放电频率(RPF)等的影响。结果与正常对照组相比,缺血缺氧组心室流出道细胞动作电位的APD50和APD90、APD均明显缩短(P<0.05),VDD及RPF显著变慢(P<0.01),并出现心律不齐;在缺血缺氧灌流液中加入炙甘草汤可明显延缓APD50、APD90和APD(P<0.05),并使VDD及RPF逐渐加快,在灌流后20min时自发放电频率基本恢复正常的节律。结论缺血缺氧可明显影响豚鼠左心室流出道自律性电活动,使其自律性发生改变,而炙甘草汤可拮抗缺血缺氧诱发的心律失常,提示炙甘草汤对治疗缺血缺氧导致的左心室流出道慢反应自律细胞的异常电生理所诱发的心律失常有显著疗效。
Objective To explore the electric activities of left ventricular outflow tract induced by hypoxia and ischemia, and to investigate the antiarrhythmic effect of Zhigancao Decoction. Methods Conventional intracellular microelectrode technique was used to record the electrophysiological parameters of spontaneous slow action potential duration （APD）, 50 % of APD （APD50） and 90 % of APD （APD50）, automatic depolarization velocity （VDD） and rate of potential frequency （RPF） in guinea pigs left ventricular outflow tract pacemaker cells of normal control group, ischemia - hypoxia group and ischemia - hypoxia adding Zhigancao Decotion group. Results Compared with normal controls, action potentials of APD50, APD90 and APD of ventricular outflow tract in ischemia - hypoxia group were significantly shortened （ P 〈 0.05）, VDD and RPF significantly slowered （ P 〈 0.01 ）, and there existed arrhythmia. The addition of Zhigan- cao Decotion can significantly prolong APD50, APD90 and APD, accelerate VDD and RPF （ P 〈 0.05）. Twenty minutes after reperfusion, spontaneous discharge frequency recovered to the normal control rhythm. Conclusion The result indicates that the automaticity of left ventricular outflow tract can be influenced by hypoxia and ischemia, and Zhigancao Decoction can counteract arrhythmia induced by ischemia and hypoxia, suggesting that the therapeutic mechanism of Zhigancao Decoction for the treatment of arrhythmia induced by ischemia and hypoxia is related to the regulation of the slow response of left ventricular outflow tract abnormalities.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第2期102-105,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
河北省科学技术研究与发展计划项目(07276416)
关键词
心室流出道
电生理
心律失常
缺血缺氧
Ventricular outflow tract
Electrophysiology
Arrhythmia
Hypoxia and ischemia
