摘要
对传统的CTAB与SDS法进行改良与简化，从茶树嫩梢部位提取高质量的基因组DNA。结果表明，用该法提取的DNA的OD260／OD280在1．8～1．9之间，电泳与PCR都能得到清晰的图谱。
High-quality genomic DNA from leaves of tea plant was extracted by improved CTAB and SDS method. Results showed that the values of OD260/OD280 of the DNA samples obtained with the improved method were between 1.8 and 1.9, which could be determined by electrophoresis and PCR.
出处
《江西农业学报》
CAS
2008年第2期34-37,共4页
Acta Agriculturae Jiangxi
关键词
茶树
DNA
提取方法
电泳
PCR
Tea plant
DNA
Extraction method
Electrophoresis
PCR
