摘要
目的探讨血必净注射液对肝脏缺血-再灌注损伤的保护作用及其机制。方法将90只健康雄性Wistar大鼠随机分成3组:假手术组(SOG组)30只,缺血再灌注组(I/R组)30只,血必净组(XBJ组)30只。血必净组术前3d经尾静脉注射血必净注射液5mL/kg/d。于再灌注6h、12h、24h各组分别随机选取10只大鼠进行标本检测:观察动物肝脏组织病理切片;测定血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、测量肝脏组织中脂质过氧化产物丙二醇(MDA);超氧化物歧化酶(SOD)的含量。结果光镜下I/R组、XBJ组与SOG组在相同时相点比较,肝细胞水肿、变性,肝小叶结构紊乱,I/R病理改变较XBJ组严重;XBJ组与I/R组在相同时相点鄙较MDA含量显著下降,均有显著性差异(P<0.01)。结论血必净注射液对肝脏缺血-再灌注损伤具有保护作用,该作用与减少氧自由基的产生有关。
Objective To investigate the protective role and mechanism of Xuebijing injection in hepatic ischemia-reperfusion injury in rots.Methods Ninety male Wistar rats were randomly divided into sham operated control group（SOG）, ischemia-reperfusion group（I/R）and Xuebijing group（XBJ）, （30 animals in each group）. Xuebijing injection（5ml/kg/ d） was administered to animals in Xuebijing group（XBJ） for three days before operation. Six hour, twelve hour, twenty- hour after reperfusion, venous blood samples and hepatic tissue samples were taken form easemem. 10 rats were randomly choiced in each group. In addition to serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST）level were respectively measured in each group. The comem of maleic dialdehyde（MDA） and activity of Superoxide Dismutase（SOD） in liver tissue was determined. The hepatic tissue was examined under light microscopies. Results After reperfusion, serum level of ALT. AST and content of MDA in liver tissue in the group of XBJ were significantly decreased than those in the group I/R（ P 〈 0.01 ）.The histologic change of the liver tissue under light microscopies in the group I/R was more obvious than in the group of XBJ. Conclusion Xuebijing injection can protect the liver fromischemia reperfusion injury. The mechanism of Xuebijing injection improving liver function may be accmplished through attenuating the degree of oxygen free radical（OFR）.
出处
《河北职工医学院学报》
2008年第1期4-6,共3页
Journal of Hebei Medical College for Continuing Education
基金
保定市科学技术局科学研究与发展计划(编号:072F090)
关键词
血必净
缺血-再灌注
肝脏
Xuebijing
ischemia - reperfusion
liver
