摘要
采用麒麟菜(Eucheuma sp.)可溶性膳食纤维(Soluble dietary fiber,SDF)、不溶性膳食纤维(Insoluble dietary fiber,IDF)和总膳食纤维(Total dietary fiber,TDF)为研究对象,分别通过体外模拟哺乳类动物胃和小肠生理pH环境条件和体内吸附实验,研究麒麟菜膳食纤维对有益金属钙、铁、锌吸附作用的影响。体内吸附实验以SD大鼠为实验动物,以混饲法给大鼠喂食膳食纤维饲料(含膳食纤维10%),实验期为5周。结果表明:(1)在体外模拟实验中,麒麟菜膳食纤维对有益金属离子Ca2+、Fe2+、Fe3+、Zn2+的吸附效果与pH、膳食纤维的可溶性和金属离子种类有关。在小肠环境中的吸附能力大于胃环境;IDF吸附能力最强,其次为TDF,SDF吸附能力最弱;各膳食纤维对Ca2+吸附作用最强,其次是Fe2+和Fe3+,最弱是Zn2+。(2)在动物体内实验中,各麒麟菜膳食纤维饲喂组动物的血清钙、血清锌和血清铁的含量随饲养时间略有增长,但与正常对照组无显著性差异(P>0.05)。因此认为,麒麟菜可溶性膳食纤维、不溶性膳食纤维和总膳食纤维对有益金属离子Ca2+、Fe2+、Fe3+和Zn2+具有不同程度的体外吸附作用,但对动物体内的钙、锌、铁元素的正常生理水平无显著影响。
It was largely reported both in domestic and overseas studies that the dietary fiber had absorption effect on some toxicants and injurants such as heavy metals, estrogen and radical, etc, so the dietary fiber had the functions of preventing and curing some diseases. But it is still unknown whether the dietary fiber can also absorb some healthful metals along with absorbing toxicants and injurants, and finally lead to malnutrition. In this study, three dietary fibers which were soluble dietary fiber （SDF）, insoluble dietary fiber （IDF） and total dietary fiber （TDF） were extracted from Eucheuma sp. and their absorption effects on the healthful metals of calcium, iron and zinc were studied in vivo and in vivo. In vitro, the experiment was carried out under simulated temperature and pH in mammalian stomach and small intestine. In vivo, SD rats were fed with feedstuff containing 10% dietary fiber for 5 weeks. The results showed that： （1）In vitro, the absorption effects of the three dietary fibers on Ca^2＋, Fe^2＋, Fe^3＋ and Zn^2＋ were related with pH, sort of metal ions and solubility of dietary fiber. The absorption effect of dietary fiber on healthful metals in small intestine was stronger than that in stomach.The absorption effect of dietary fiber on Ca^2＋ was the strongest, followed by Fe^2＋, Fe^3＋, and Zn^2＋ orderly, Among the three dietary fibers, the absorption effect of IDF on these healthful ions was the highest, TDF second and the SDF last. （2）In vivo, the quantity of healthful metals in serum of trial rats increased slightly with the feeding time, but had no significant difference with the control （P 〉 0.05）. These results indicate that SDF, IDF and TDF from Eucheuma sp. have absorption effects on Ca^2＋, Fe^2＋, Fe^3＋ and Zn^2＋ in vitro, while they have no significant effects on physiological levels of calcium, iron and zinc in animal serum.Therefore, it could be concluded that dietary fiber probably has no significantly negative effect on people＇ s assimilating healthful metals of calcium, iron and zinc. [Journal of Fishery Sciences of China, 2008, 15 （2）： 323-329 ]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第2期323-329,共7页
Journal of Fishery Sciences of China
基金
广东省科技计划项目(2006B20401007)
中央级公益性科研院所专项基金项目(20072ZD06)
关键词
麒麟菜
膳食纤维
钙
锌
铁
Eucheuma
dietary fiber
calcium
iron
zinc
