摘要
采用3H-TdR同位素示踪法研究了河流弧菌(Vibrio fluvialis)TS-1对青石斑鱼(Epinephelus awoara)表皮黏液的黏附作用。研究结果显示,经TSB培养的河流弧菌对青石斑鱼表皮黏液的黏附作用极显著高于经TSA培养的河流弧菌(P<0.01);河流弧菌经TSB培养后能很好地黏附于青石斑鱼表皮黏液,其黏附量随菌浓度升高而增大并符合饱和黏附动力学特征;不同生长阶段河流弧菌的黏附能力不同,在培养初期阶段细菌的黏附量先是随着培养时间的延长而增大,并在培养24h后黏附量达到最大,而后随培养时间的延长其黏附量急剧下降。本实验研究结果表明,河流弧菌对青石斑鱼表皮黏液有较强的黏附作用,其黏附作用受细菌培养条件、营养状况等自身因素的显著影响。本研究结果有助于了解河流弧菌的流行病学和致病机理。
Adhesion of Vibrio fluvialis to skin mucus of Epinephelus awoara had been investigated by using [methyl-^3H] thymidine as the isotope tracer. The results show that the adhension ability of Vibrio fluvialis incubated in TSB is extremely stronger than that of V. fluvialis incubated on TSA （P〈0.01）. The adhension quantity of V.fluvialis to skin mucus of E. awoara increases with the densities of bacterial suspension, and the bacterial adhension measures up to the saturation kinetics. After the bacteria were treated with rabbit antiserum against O-antigen of Efluvialis, the number of adhering bacteria reduced by 52%. V.fluvialis at different culture stages showed different adhension ability. At early growth stage, the number of adhering bacteria increased with incubation time and reached peak at 24 h, and then droped sharply with the culture time after 24 h. The results indicate that V.fluvialis adheres to the skin mucus ofE. awoara strongly by specific adhesion, and this kind of bacterial adhension is remarkably influenced by culture conditions and nutrition level. These results are helpful for better understanding the characteristics of epidemiology and pathogenesis of V.fluvialis. [Journal of Fishery Sciences of China, 2008, 15 （2） ： 347-351]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第2期347-351,共5页
Journal of Fishery Sciences of China
基金
国家863计划项目(2007AA09Z115)
福建省科技项目(2006F5066)
福建省高校水产科学技术与食品安全重点实验室开放基金(2007J101)
关键词
河流弧菌
青石斑鱼
体表黏液
黏附
Vibriofluvialis
Epinephelus awoara
skin mucus
adhesion
