摘要
目的观察高血压病患者高密度脂蛋白抗氧化活性有无变化及可能的影响因素。方法纳入33名高血压病患者及32名健康对照者,采用一次性密度梯度超速离心法分离高密度脂蛋白及低密度脂蛋白,体外Cu2+诱导法作脂蛋白氧化,用分光光度法、硫代巴比妥酸法测定高密度脂蛋白抗氧化能力、氧化延迟时间和体外过氧化程度,分光光度仪连续监测法测定血清对氧磷酶1活性。结果高血压病患者与正常对照组比较,高密度脂蛋白抗氧化活性及对氧磷酶1活性下降(P均<0.05),高密度脂蛋白氧化延迟时间、体外过氧化程度差异没有统计学意义;高密度脂蛋白抗氧化活性与对氧磷酶1活性呈正相关,与收缩压、舒张压和总胆固醇浓度呈负相关(r分别为0.317、-0.387、-0.42和-0.259,P均<0.05)。结论高血压病患者高密度脂蛋白抗氧化活性降低可能是易患动脉粥样硬化的重要因素。血压、对氧磷酶1活性等影响高密度脂蛋白抗氧化活性。
Aim To compare the differences of high density lipoprotein（HDL） antioxidant activity between normal people and the essential hypertension patients,and to explore the factors that may affect the antioxidant capacity of HDL. Methods Thirty-three patients with essential hypertension and 32 normal people were included.HDL and low density lipoprotein（LDL） were isolated by one-step density grandient ultracentrifugation,and induced oxidation with external Cu 2＋.Antioxidant activity of HDL,lag time,lipid peroxidation degree were determined by spectrophotometric and thiobarbituric acid reactive substances methods.Paraoxonase 1（PON1） activity was measured with continuous monitoring using phenylacetate as substrate.Results In essential hypertension patients,the inhibitory effect of HDL on LDL oxidation and the activity of PON1 were reduced（P〈 0.05）,the lag time of oxidation and the lipid peroxidation degree did not show statistically significant difference.HDL antioxidant capacity was positively correlated with PON1 activity and negatively correlated with systolic pressure,diastolic pressure,and total cholesterol level（with r= 0.317,-0.387,-0.42,-0.259 respectively,P〈 0.05）.Conclusions HDL antioxidant activity may be one of the most important determinants for the development of atherosclerosis in patients with essential hypertension.The impaired HDL antioxidant activity is affected by high levels of blood pressure,and low activity of PON1.
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第12期909-912,共4页
Chinese Journal of Arteriosclerosis
基金
四川省科技攻关项目资助课题(07KJT-05)
关键词
内科学
高血压病
高密度脂蛋白
低密度脂蛋白
脂质氧化
抗氧化
动脉粥样硬化
Essential Hypertension
High Density Lipoproteins
Low Density Lipoprotein
Lipid Peroxidation
Antioxidant
Atherosclerosis
