摘要
目的观察肝硬化大鼠肠道细菌过度生长及细菌易位情况,研究微生态制剂对其影响。方法皮下注射40%CCl4橄榄油溶液诱导大鼠肝硬化模型,微生态制剂治疗。随机分成正常对照组(13只)、肝硬化模型组(10只)、肝硬化益生元治疗组(19只)、肝硬化益生菌治疗组(19只)及肝硬化治疗对照组(20只)5组。检测各组大鼠肠杆菌计数、血清内毒素,无菌操作取肠系膜淋巴结、肝、脾组织分别进行细菌培养观察细菌易位情况。结果①肝硬化模型组大鼠小肠内肠杆菌计数较正常组明显增多(P<0.01);内毒素血症的阳性率及细菌易位率明显高于正常组(P<0.01);②益生元治疗组肠杆菌计数较肝硬化治疗对照组明显降低(P<0.05)。益生元和益生菌治疗组内毒素血症的阳性率及细菌易位率均较肝硬化治疗对照组降低(P<0.05),但二者之间无明显差异。结论①肝硬化大鼠存在小肠内肠杆菌明显过度生长,内毒素血症阳性率及细菌易位率明显高于正常组;②益生元和益生菌在治疗肝硬化大鼠内毒素血症及防止肠道细菌易位方面有明显疗效;③益生元对肝硬化大鼠肠杆菌过度生长有抑制作用。
Objective To study the intestinal bacterial overgrowth （IBO） and bacterial translocation （BT） in cirrhotic rats , and the effect of microecologics on it. Methods In order to induce cirrhosis model the rats were given 40% CC14 elaeo - solution by subcuta-neous injection, then were treated by microecologics （prebiotics and probiotics ）. The rats were randomly divided into normal control group （ 13 rats） , cirrhosis model group （ 10 rats） , prebiotics therapy group （ 19 rats） , probiotics therapy group （ 19 rats） and therapy control group（20 rats） . To detect endotoxin, cultivate and count enteric bacilli, mesenteric lymph nodes, tissue of liver and spleen were taken to cultivate bacteria. Results 1. the counts of small intestinal bacilli in cirrhosis rats were obviously higher than normal rats （ P 〈 0. 01 ） ,the positive rate of endotoxemia and BT were also obviously higher than the rats in normal group（ P 〈 0.01 ）. 2. the counts of small intestinal bacilli in prebiotics therapy group were obviously lower than that in therapy control group （ P 〈 0.05 ） . In prebiotics and probiotics therapy groups, the positive rate of endotoxemia and BT were all obviously lower than the rats in cirrhosis and therapy control group （P 〈 0.05）, but the difference between the two therapy groups does not exist. Conclusions The cirrhotic rats have IBO, and their positive rate of endotoxemia and BT were all obviously higher than normal rats, 2,Prebiotics and probiotics have significant effect in treating endotoxemia and preventing BT in cirrhosis rats, but no significant difference between the two drugs. 3.Prebiotics can inhibit small intestinal bacilli from overgrowth.
出处
《医学研究杂志》
2008年第3期78-80,共3页
Journal of Medical Research
关键词
肝硬化
小肠细菌过度生长
内毒素
细菌易位
微生态制剂
Cirrhosis of liver
Intestinal bacterial overgrowth
Endotoxin
Bacterial translocation
Microecologics
