摘要
为了解原发性食管癌组织中ＧＳＴπ基因的表达情况，用ＲＴＰＣＲ方法定量检测了食管癌组织及相对应的正常食管粘膜组织中ＧＳＴπ基因的表达。结果：在癌组织中ＧＳＴπ基因表达的阳性率为８０．０％，在癌旁正常粘膜组织中为８６．６％。但在癌组织中ＧＳＴπ基因表达的强度大于正常粘膜组织。食管癌组织分化越差，ＧＳＴπ基因阳性表达率越低，Ｐ＜０．０５。结果提示：在原发性食管组织中ＧＳＴπ基因存在较高频率的阳性表达，在食管癌的发生发展中可能起着重要作用。
In order to demonstrate the alterations of glutathione S transferase π gene in primary human esophageal carcinoma,quantitative RT PCR(Reverse Transcription Polymerase Chain Reaction,RT PCR) was employed to detect the expression of GST π gene.The results showed that the expression of GST π gene was observed in 80.0% of 30 specimens of carcinoma and in 86.6% of 30 specimens of normal esophageal mucosa,but the intensity of expression in cancerous tissues was stronger than that in normal mucosa.The expression of GST π gene was associated with the differentiation of esophageal carcinoma.The poorer the differentiation,the lower the positive expression rate and vice versa.It is suggested that the expression of GST π gene presented at high frequency in human esophageal carcinoma might play a key role in the cacinogenesis of esophagus.
出处
《河南医科大学学报》
1997年第3期22-24,共3页
Journal of Henan Medical University
基金
国家"八五"科技攻关项目
河南省重大科技攻关项目
关键词
食管肿瘤
谷胱甘肽
S-转移酶
RT-PCR
esophageal carcinoma,glutathione S transferase π,reverse transcription polymerase chain reaction
