摘要
Two cellobiohydrolases from Trichoderma pseudokiningii S-38 were isolatde and pruified by DEAE-Sephadex A-25 ion exchange and gel filtration chromatography. The molecular weights of the purified enzymes were estimated to be 66 000 for cellobiohydrolase Ⅰ and 67 000 for cellobiohydrolase Ⅱ respectively, by 10% SDS-PAGE. Some propertied of the purified enzymes were also determined.
Two cellobiohydrolases from Trichoderma pseudokiningii S-38 were isolatde and pruified by DEAE-Sephadex A-25 ion exchange and gel filtration chromatography. The molecular weights of the purified enzymes were estimated to be 66 000 for cellobiohydrolase Ⅰ and 67 000 for cellobiohydrolase Ⅱ respectively, by 10% SDS-PAGE. Some propertied of the purified enzymes were also determined.
基金
国家自然科学基金!39430020
国家教委博士基金
关键词
外切葡聚糖
纤维二糖水解酶
分离
纯化
性质
β-1, 4-Glucan-cellobiohydrolases, Cellulase, Purification, Trichoderma pseudokiningii S-38
