摘要
从提高虾青素产量和降低生产成本综合考虑。研究选择了混合碳源、混合氮源、柠檬酸铵((NH_4)_3C_6H_5O_7)、Na_2HPO_4、K_2HPO_4、MgSO_4·7H_2O组成培养基。正交设计优选出的培养基为混合碳源(糖蜜40%、淀粉塘60%)30 g/L、混合氮源(玉米浆40%、(NH_4)_2SO_460%)7 g/L、MgSO_4·7H_2O 1.5 g/L、(NH_4)_3C_6H_5O_7 2g/L、Na_2HPO_4 2.0 g/L,用此优化培养基摇瓶培养红发夫酵母获得生物量16.92 g/L,虾青素含量903μg/g和虾青素产量15 279μg/L。
To the end of achieving the cost efficiency of astaxanthin production, we selected simple and inexpensive media components for the study introducd in this paper. Using orthogonal designs L25 (5^6 ), the optimal media for flask cultivation was obtained: compound carbon sources 30g/L, compound nitrogen sources 7 g/L, (NH4)3C6H5O7 2 g/L, Na2HPO4 2.0g/L, MgSO4 · 7H2O 1.5 g/L. Using this optimized media, the maximum biomass and the astaxanthin yield were 16.92 g/L and 15 279μg/L separately.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第2期61-64,共4页
Food and Fermentation Industries
基金
广东省科技攻关项目(2002C1040101)
广州市科技计划项目(2003Z2-E0131)
关键词
红发夫酵母
虾青素
培养基优化
Pha f fia rhodozyma, astaxanthin,media optimization