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机械刺激诱导的烟草悬浮培养细胞耐热性及其与过氧化氢的关系 被引量：9

Mechanical Stimulation-induced Heat Tolerance of Suspension Culture Cells in Tobacco(Nicotiana tobacum L.)and Its Relation to H_2O_2
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摘要 机械刺激可诱发烟草悬浮培养细胞中H_2O_2的积累,提高烟草悬浮培养细胞在高温胁迫下的存活率和再生能力,缓解高温胁迫下的细胞活力丧失和膜伤害,外源H_2O_2预处理也可提高烟草悬浮细胞的耐热性。这些暗示H_2O_2作为信号分子可触发机械刺激诱导的烟草悬浮细胞耐热性形成。 Mechanical stimulation （MS） induced H2O2 accumulation in tobacco suspension culture cells, increased survival percentage and regrowth of tobacco suspension cells under high temperature at 43 ℃, and alleviated the loss of cell vitality and membrane damage. In addition, exogenous H2O2 pretreatment also significantly enhanced heat tolerance in tobacco suspension cells. These results suggested that H2O2 could act as a signaling molecule to induce the formation of MS-induced heat tolerance in tobacco suspension cells.
作者 李忠光 龚明
机构地区 云南师范大学生命科学学院
出处 《植物生理学通讯》 CAS CSCD 北大核心 2008年第1期42-44,共3页 Plant Physiology Communications
基金 国家自然科学基金(30460016) 云南省自然科学基金(2006C0030M) 云南省教育厅基金(06Y117B)。
关键词 烟草悬浮培养细胞 机械刺激 耐热性 H2O2 tobacco suspension cells mechanical stimulation heat tolerance H2O2
分类号 Q943.1 [生物学—植物学]
  • 相关文献

参考文献18

  • 1李忠光,杜朝昆,龚明.Ca^(2+)和钙调素对H_2O_2诱导的玉米幼苗耐热性的调控[J].植物生理与分子生物学学报,2005,31(5):515-519. 被引量：14
  • 2李忠光,宋玉泉,龚明.二甲酚橙法用于测定植物组织中的过氧化氢[J].云南师范大学学报（自然科学版）,2007,27(3):50-54. 被引量：42
  • 3李忠光,杨仕忠,周滔,龚明.烟草悬浮培养细胞的建立及其对机械刺激的敏感性研究[J].云南师范大学学报（自然科学版）,2005,25(6):43-45. 被引量：13
  • 4Braam J (2005). In touch: plant responses to mechanical stimuli. New Phytol, 165:373-389
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  • 6de Pinto MC, Francis D, de Gara L (1999), The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells. Protoplasma, 209:90-97
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二级参考文献20

  • 1李忠光,龚明.抗氧化系统在H_2O_2诱导的玉米幼苗耐热性形成中的作用[J].植物生理学通讯,2003,39(6):575-579. 被引量：10
  • 2李忠光,龚明.植物中超氧阴离子自由基测定方法的改进[J].云南植物研究,2005,27(2):211-216. 被引量：157
  • 3李忠光,杜朝昆,龚明.Ca^(2+)和钙调素对H_2O_2诱导的玉米幼苗耐热性的调控[J].植物生理与分子生物学学报,2005,31(5):515-519. 被引量：14
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  • 7Prasad TK, Andrson MD, Martin BA et al. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide[J]. Plant Cell, 1994, 6:65 ～ 74.
  • 8Gong M, Chen B, Li ZG et al. Heat-shock-induced cross adaptation to heat, chilling, drought and salt stress in maize seedlings and involvement of H2O2[J]. J Plant Physiol. 2001, 158:1125 ～ 1130.
  • 9Legendre L, Rueter S, Heinstein PF et al. Characterization of the oligogalacturonide-induced oxidative burst in cultured soybean (Glycine max) cells[J]. Plant Physiol, 1993, 102:233-240.
  • 10Neill SJ,Diskan R,Hancock J8T.Nitric oxide signalling in plants[J].New Phytol,2003,159:11-35.

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植物生理学通讯
2008年 第1期

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