摘要
为了探讨兔斯氏艾美耳球虫(E.stiedai)感染时试验家兔肝的诱导型一氧化氮合酶(iNOS)活性和血清中NO浓度变化以及NO对兔球虫的抑制或杀伤作用,通过腹腔注射的途径给予感染E.stiedai的家兔不同浓度的NOS底物L-精氨酸(L-Arg)、NO供体硝酸甘油(GTN)和iNOS抑制剂L-氨基胍(L-AG),利用NOS活性测试盒测定肝的iNOS活性,用硝酸还原酶法测定血清中NO的浓度,采用麦克马斯特氏法计数每克粪便的球虫卵囊数(OPG),根据试验兔肝的剖检和镜下病变来进行病变计分。结果表明:感染E.stiedai后,家兔肝匀浆的iN-OS活性和血清中NO浓度逐渐升高,感染对照组、添加L-Arg组、GTN组、L-AG组4组均在感染后第12 d达到最高值,而后逐渐下降,于23 d左右下降到感染前的水平;L-Arg可增强肝的iNOS活性,使其释放大量NO,产生对球虫的抑制或杀伤作用,减轻球虫感染引起的病变;硝酸甘油可在兔体内释放NO,对球虫产生一定的抑制作用,稍减轻球虫感染引起的病变;相反,L-AG则抑制肝的iNOS活性,使生成的NO减少,对球虫的抑制作用减弱甚至消失,从而加重球虫感染引起的病变。结果提示:NO及iNOS确实参与了家兔E.stiedai的感染过程,并且NO在球虫感染过程中起到了抑制或杀伤球虫的作用。
To probe into the activity of inducible nitric oxide synthase （iNOS） in the liver , the concentration of nitric oxide （NO） in the serum and its inhibition or killness on coccidia of rabbits, the rabbits infected with Eimeria stiedai were injected with the substrate of nitric oxide synthase-L - arginine （ L - Arg） width different concentrations, the nitric oxide donor-nitroglycerin （GTN） and the inhibitor of inducible nitric oxide synthase-L -aminoguanidine （ L - AG） through the abdominal cavity. Then messure the activity of inducible nitric oxide synthase in the liver using test box of nitric oxide synthase activity. Messure the concentration of nitric oxide in the serum via the method of nitric acid reductase. Count the coccidian oosysts per gram feces （OPG） using McMaster＇s method. Score the pathologies according to anatomic structures of rabiits＇livers and micoscopic pathological changes. The results showed that： after infected with Eimeria stiedai, the activity of inducible nitric oxide synthase of rabbits＇liver chips and the concentration of nitric oxide in the serum increased gradually. The control group, L - arginine group, nitroglycerin group and L -aminoguanidine group reached the top 12 days after infected, and then decreased gradually and reached the level before infected at about 23days; L -arginine can enhance the activity of inducible nitric oxide synthase in the liver and make it release large amount of nitric oxide, so that inhib-it or kill Eimeria stiedai and ease the pathologies caused by Eimeria stiedai; Nitroglycerin can release nitric oxide in vivo and inhibit Eimeria stiedai, so that ease the pathologies. On the contrary, L - aminoguanidine inhibits inducible nitric oxide synthase in the liver and reduces nitric oxide produced, so that the inhibition on Eimeria stiedai was weakened or even not existed andaggravated the pathologies. The results clues on that nitric oxide and inducible nitric oxide synthase indeed participate in the process of rabbits＇infection with Eimeria stiedai and nitric oxide plays the role of inhibition or killing in the process of infection.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2008年第1期53-58,共6页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省自然科学基金资助项目(Y2003D04)
