摘要
采用３Ｈ－ＴｄＲ参入法，测定碱性成纤维细胞生长因子（ｂＦＧＦ）、胰岛素和内皮素－１（ＥＴ－１）对体外培养的大鼠肾小球系膜细胞（ＭＣ）增殖的影响，以及胰岛素与ｂＦＧＦ或ＥＴ－１促ＭＣ增殖的协同作用。结果表明，不同浓度的ｂＦＧＦ（５－２００ｎｇ／ｍｌ）和胰岛素（０．１－２．４Ｕ／ｍｌ）均显著升高ＭＣ的３Ｈ－ＴｄＲ参入值（ｃｐｍ值）（Ｐ＜０．０１）。ＥＴ－１对ＭＣ的ｃｐｍ值的影响依剂量不同呈现两种不同的效应，在１０－９－１０－７ｍｏｌ／Ｌ时，随着浓度的升高，ＭＣ的ｃｐｍ值明显升高（Ｐ＜０．０１），并以１０－８ｍｏｌ／Ｌ作用最强；当升高到１０－６ｍｏｌ／Ｌ时，ＭＣ的ｃｐｍ值出现降低趋势。胰岛素与ｂＦＧＦ或低浓度ＥＴ－１（≤１０－８ｍｏｌ／Ｌ）共同作用于ＭＣ时，ＭＣ的ｃｐｍ值明显高于二者单独作用之和（Ｐ＜０．０１），与高浓度ＥＴ－１（＞１０－７ｍｏｌ／Ｌ）共同作用于ＭＣ时，ＭＣ的ｃｐｍ值小于二者单独作用之和（Ｐ＞０．０５）。上述结果说明，胰岛素、ｂＦＧＦ和ＥＴ－１均能显著促进ＭＣ增殖；胰岛素与ｂＦＧＦ或低浓度的ＥＴ－１促ＭＣ增殖具有正协同作用，与高浓度ＥＴ－１呈现负协同作用。
The effects of basic fibroblast growth factor(bFGF),insulin and endothelin 1 (ET 1) on proliferation of rat glomerular mesangial cells(MC) cultured in vitro and the synergetic effects of insulin and bFGF or ET 1 on MC proliferation were determined by 3 H TdR incorporation experiment.The results showed that bFGF at concentrations of 5-200ng/ml and insulin at concentrations of 0 1-2 4U/ml significantly increased the value of 3 H TdR incorporated into MC(the cpm value). The effect of ET 1 on the cpm value was different at different doses. At concentration ranging from 10 9 mol/L to 10 7 mol/L, the cpm value significantly increased with increasing the concentration of ET 1,and the highest value was at concentration of 10 8 mol/L, However,the cpm value decreased at concentration of 10 6 mol/L ET 1. When MCs were incubated simultaneously with insulin and bFGF or lower concentration of ET 1(<10 8 mol/L), the cpm value was significantly higher than the sum of that induced independently by insulin and bFGF or ET 1.But the joint effect of insulin and higher concentration of ET 1(>10 7 mol/L) on the cpm value was lower than the sum of that induced separately by insulin and ET 1.It was indicated that bFGF, insulin and ET 1 significantly stimulated MC proliferation; insulin exhibited positive synergetic effect with bFGF or lower concentration of ET 1 on MC proliferation, negative synergetic effect with higher concentration of ET 1 on MC proliferation.
出处
《中国应用生理学杂志》
CAS
CSCD
1997年第2期159-162,共4页
Chinese Journal of Applied Physiology
基金
河北省自然科学基金
