摘要
为了得到优良的草莓抗病植株，采用根癌农杆菌介导法进行几丁质酶基因（chit42）对草莓叶片的转化，对转化过程中的一些因素进行研究，并得到了转化最优方案：乙酰丁香酮AS浓度80-120μmol／L，10-20min侵染，3d共培养．经PCR检测初步鉴定外源基因已经导入．
With the transformation strawberry leaves, transgenic strawberry was obtained through the chit42 gene transformation by Agrobacterium tumefactions, so that disease-resistance transgenic plants were required. Factors on Agrobacterium tumifaciens-mediated gene transfer was discussed in details. We preliminarily determined an effective system for genetic transformation. The protocol was follows： AS 80-120 μtmol/L as co-culture medium, 10-20 minutes for infection, 3 days for co-culture. The regenerated kanamycin resistant plants were analyzed by PCR. The result indicated that the chit42 gene has been integrated into the strawberry.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期25-28,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖北省教育厅项目(Q200526002)
关键词
草莓
几丁质酶基因
转化
strawberry
chitinase gene
transformation
