摘要
在ＳｐｈｅｒｉｓｏｒｂＣ８柱上，甲醇—水相（取磷酸二氢钾４ｇ、三乙胺２．５ｍＬ，辛烷基磺酸钠１ｇ，加水至１０００ｍＬ，磷酸调ｐＨ至２．５）（６６３４）为流动相，高效液相色谱法同时分离和测定复方康泰唑冲洗液中甲硝唑、醋酸氯己定和硝酸咪康唑。考察了色谱体系中各因素对色谱保留的影响。３组分的线性关系良好，回收率满意。
A HPLC method is described for the determination of metronidazole,chlorhexidine acetate and miconazole nitrate in Kangtaizuo washing solution on a C 8 column using methanol-buffer solution(66∶34)as mobile phase.The buffer solution contains 4g potassium dihydrogen phosphate,2 5ml triethylamine,1g sodium 1 octanesulfonate in 1000mL water solution and adjust to pH2 5 with phosphoric acid.The influence of column packing,pH,triethylamine and sodium 1 octanesulfonate on chromatographic behavior of the three compounds is also discussed.Assay results are satisfactory using this proposed method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1997年第1期9-11,共3页
Chinese Journal of Pharmaceutical Analysis
