摘要
目的:研究格尔德霉素(GDM)对肝细胞生长因子(HGF)引起的胶质瘤细胞增殖及相关基因表达的影响。方法:选用人恶性胶质瘤细胞系U251MG和U87MG,应用HGF作用24h后,分别加入不同浓度的GDM再处理48h。MTT法检测细胞增殖及抑制,分组为:正常细胞组、HGF组、GDM组、HGF+GDM组和紫杉醇组,其中HGF为终浓度30μg.L-1,GDM为50、250、500与1000nmol.L-1,紫杉醇为60μg.L-1;RT-PCR法检测HGF及c-Met基因的表达,分组如上,GDM为1000nmol.L-1。结果:HGF作用U251MG与U87MG细胞24h后,细胞增殖率分别为0.139±0.070与0.242±0.167,而50、250、500与1000nmol.L-1GDM对U251MG和U87MG生长具有抑制作用,抑制率分别为0.029±0.028、0.027±0.017、0.312±0.084和0.339±0.047与0.116±0.069、0.222±0.191、0.269±0.056和0.276±0.031;而紫杉醇对U251MG和U87MG细胞的抑制率分别为0.075±0.062与0.071±0.044;RT-PCR结果显示,HGF组HGF与c-Met表达较正常组增加(P<0.05),而HGF+GDM组则较HGF组明显降低(P<0.05)。结论:GDM能抑制HGF诱导的胶质瘤细胞增殖,并且能从基因水平抑制其表达。
Objective To investigate the effects of geldanamycin （GDM） on the proliferation and relative gene expression in glioma cells induced by hepatocyte growth factor （HGF） . Methods U251 MG and U87 MG of human malignant glioma cells were cultivated by cell culture technique. After cultivated in the presence of HGF for 24 h, U251 MG and U87 MG cells were cultivated for another 48 h in the presence of GDM at different concentrations （50, 250, 500, and 1 000 nmol ·L^-1 ）. The inhibitory rates of growth were examined by MTT essays. The experiment was divided into normal cells, cells＋ HGF （30 μg·L^-1）, cells＋ GDM （50, 250, 500 and 1 000 nmol ·L^-1） and cells＋ HGF＋ GDM and paclitaxel groups. The relative gene mRNA expression level was detected with RT-PCR methods. Results After U251 MG and U87 MG cells were treated with HGF for 24 h, the proliferative rates were 0. 139±0.07 and 0. 242±0. 167, respectively; and 50, 250, 500 and 1 000 nmol ·L^-1 GDM had inhibitory effects on U251 and U87 cells, the inhibitory rates were 0. 029±0. 028, 0. 027±0. 017, 0.312±0.084, 0. 339±0. 047 and 0. 116±0.069, 0.222±0.191, 0.269±0.056, 0.276±0.031; the inhibitory rates of paclitaxel on U251 MG and U87 MG cells were 0. 075±0. 062 and 0. 071±0. 044. The results of RT-PCR appered that the HGF and c-Met mRNA in U251 MG and U87 MG cells in HGF group were lower than those in normal group （ （P〈0.05）, but the HGF and c-met mRNA in U251 MG and U87 MG cells in HGF＋GDM group were lower than those in HGF group （P〈0.05）. Conclusion GDM could inhibit the proliferation of glioma cells induced by HGF and regulate the relative gene expression.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第2期199-203,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题(20030423-03)
