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Early apoptotic changes in human spermatozoa and their relationships with conventional semen parameters and sperm DNA fragmentation 被引量:12

Early apoptotic changes in human spermatozoa and their relationships with conventional semen parameters and sperm DNA fragmentation
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摘要 Aim: To investigate whether early apoptotic changes in spermatozoa can be significant markers for sperm quality. Methods: Two early apoptotic changes in the semen of 56 men were assessed using Annexin V (AN)/propidium iodide (PI) staining for phosphatidylserine externalization and JC-1 staining for mitochondrial membrane potential (MMP). The results were compared with conventional semen parameters and DNA fragmentation identified using the TUNEL assay. Results: The different labeling patterns in the bivariate Annexin V/PI analysis identified four distinctive spermatozoa populations. The percentage of AN^-/PI^- spermatozoa positively correlated with conventional semen parameters and MMP, but negatively correlated with TUNEL (+) spermatozoa. As for the AN^-/PI^+ fraction, we found an opposite result in comparison to AN^-/PI^- spermatozoa. The level of early apoptotic AN^+/PI^- spermatozoa negatively correlated with MMP and sperm motility. The level of late apoptotic AN^+/PI^+ spermatozoa negatively correlated with conventional semen parameters and MMP, and positively correlated with TUNEL (+) spermatozoa. MMP positively correlated with conventional semen parameters, but negatively correlated with TUNEL (+) spermatozoa. Conclusion: Although early apoptotic AN^+/PI^- spermatozoa only negatively correlates with sperm motility, the differences in proportion of each subpopulation of spermatozoa (especially, the percentage of AN^-/PI^- spermatozoa), and decreased MMP might be significant markers for diagnosing male infertility. They possibly bring additional information to predict the outcome of in vitro fertilization. (Asian J Androl 2008 Mar; 10: 227-235) Aim: To investigate whether early apoptotic changes in spermatozoa can be significant markers for sperm quality. Methods: Two early apoptotic changes in the semen of 56 men were assessed using Annexin V (AN)/propidium iodide (PI) staining for phosphatidylserine externalization and JC-1 staining for mitochondrial membrane potential (MMP). The results were compared with conventional semen parameters and DNA fragmentation identified using the TUNEL assay. Results: The different labeling patterns in the bivariate Annexin V/PI analysis identified four distinctive spermatozoa populations. The percentage of AN^-/PI^- spermatozoa positively correlated with conventional semen parameters and MMP, but negatively correlated with TUNEL (+) spermatozoa. As for the AN^-/PI^+ fraction, we found an opposite result in comparison to AN^-/PI^- spermatozoa. The level of early apoptotic AN^+/PI^- spermatozoa negatively correlated with MMP and sperm motility. The level of late apoptotic AN^+/PI^+ spermatozoa negatively correlated with conventional semen parameters and MMP, and positively correlated with TUNEL (+) spermatozoa. MMP positively correlated with conventional semen parameters, but negatively correlated with TUNEL (+) spermatozoa. Conclusion: Although early apoptotic AN^+/PI^- spermatozoa only negatively correlates with sperm motility, the differences in proportion of each subpopulation of spermatozoa (especially, the percentage of AN^-/PI^- spermatozoa), and decreased MMP might be significant markers for diagnosing male infertility. They possibly bring additional information to predict the outcome of in vitro fertilization. (Asian J Androl 2008 Mar; 10: 227-235)
出处 《Asian Journal of Andrology》 SCIE CAS CSCD 2008年第2期227-235,共9页 亚洲男性学杂志(英文版)
基金 Acknowledgment This work was supported by grants from the National Natural Science Foundation of China (No. 30470703). The authors would like to thank Dr Jian- Feng Li for his valuable comments and assistance.
关键词 Annexin V apoptosis DNA fragmentation INFERTILITY MITOCHONDRIA SPERM Annexin V apoptosis DNA fragmentation infertility mitochondria sperm
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