摘要
以大豆品种北京小黑豆的成熟种子为材料,研究了不同消毒方法、激素配比、外植体以及芽诱导时间对再生频率的影响。结果表明,使用氯气消毒法,以大豆子叶节为外植体,在B5+1.0 mg.L-1 6-BA的诱导培养基上诱导5 d后,北京小黑豆能获得较高的植株再生率,每个外植体成苗数在3个左右。
Effect of different disinfect ways, hormone combination, different explants and shoot induction on soybean tissue culture efficiency were studied. Mature seeds of Peking were used as explants. The results showed chlorine gas was a better disinfection method. When the soybean cotyledonary node was induced in the medium B5＋l.0 mg·L^-1 6-BA for 5 days, the Peking could regenerate rapidly. The shoot number of every explant was about three.
出处
《东北农业大学学报》
CAS
CSCD
2008年第3期5-8,共4页
Journal of Northeast Agricultural University
基金
国际科技合作重点项目(大豆抗疫霉根腐病资源创新及主效QTL与环境互作的研究)(2005DFA30340)
大豆生物学教育部重点实验室主任基金项目(SB07A04)
关键词
大豆
子叶节
茎失
激素
再生
soybean
cotyledonary node
stem tip
hormone
regeneration
