摘要
我室以往的工作证明2Hz和100Hz电针可引起中枢释放不同种类的阿片肽，本工作试图阐明不同频率的电针是否影响三种阿片肽的基因转录。用地高辛标记的反义cRNA探针进行原位杂交，显示大鼠脑内前脑啡肽原（preproenkephalin，PPE），前强啡肽原（preprodynorphin，PPD）和前阿黑皮素原（proopiomelanocortin，POMC）mRNA。结果：（1）低、高频电针均不影响POMCmRNA的水平。（2）对PPE的影响，两种频率电针诱导脑干网状结构头端腹内侧区PPEmRNA升高的幅度相似，2Hz电针诱导视上核、视交叉上核、下丘脑室分核、弓状核、腹内侧核反外侧丘系核的PPEmRNA表达比100Hz电外高得多。（3）对PPD的表达，2Hz电针不改变脑内PPDmRNA的水平，100Hz电针可使现上核、下丘脑室旁核、腹内侧核及脑干臂旁核的PPDmRNA明显升高。鉴于肽类的加速释放必然引起合成的增加，上述结果为不同频率电针加速不同内源性阿片肽的释放和合成，从而发挥镇痛作同，提供了有力的佐证。
Previous findings from this laboratory have shown that low (2 Hz) and high (100Hz) - frequency electroacupuncture (EA) accelerated the release of different kinds of opioid peptides in the CNS. In the present study, we tried to elucidate whether EA of different frequencies would affect the transcription of genes encoding different opioid peptides. Digoxin-labeled antisense cRNA probes were used for in situ hybridization to detect the mRNA encoding preproenkephalin (PPE), preprodynorphin (PPD) and proopiomelanocortin (POMC) in the rat brain. The results showed that: (1) Neither 2 Hznor 100 Hz EA altered the POMC mRNA level in the rat brain. (2) EA of the two frequencies induced a similar degree of increase of PPE mRNA in rostromedial reticular formation (gigantocellular, paragigantocellular and lateral reticular nucleus); whereas in supraoptic nucleus, suprachiasmatic nucleus, arcuate nucleus, paraventricular hypothalamic nucleus, ventromedial nucleus and the nucleus of lateral lemniscus, 2 Hz EA induced a higher PPE mRNA expression than 100 Hz EA. (3) 100 Hz EA markedly increased the PPD mRNA levels in supraoptic nucleus, paraventricular hypothalamic nucleus, ventromedial nucleus and parabrachial nucleus, while 2 Hz was without effect. Since de novo peptide synthesis is regarded as a natural outcome following accelerated peptide release, the present results substantiate our previous observation that EA of different frequencies exert different acceleratory effects on the release and synthesis of different opioid peptides in the central nervous system.
出处
《生理学报》
CAS
CSCD
北大核心
1997年第2期121-127,共7页
Acta Physiologica Sinica
关键词
电针刺激
针刺镇痛
阿片肽
基因表达
神经生理学
electroacupuncture stimulation
stimulation frequency
preproenkephalin
preprodynorphin proopiomelanocortin
gene expression
