摘要
本文采用逆转录聚合酶链反应（RT－PCR）方法测定大鼠内毒素血症不同时期胸腰段背根神经节降钙素基因相关肽（CGRP）mRNA水平的改变，结合血浆CGRP水平的改变，以期全面了解大鼠内毒素血症不同时期CGRP释放与合成的变化。结果显示：注射内毒素（5mg／kg）后30min时，大鼠血浆CGRP开始增高，而背根神经节CGRPmRNA水平无明显变化；注射内毒素后3h时，血浆CGRP及背根神经节CGRPmRNA均明显增高．分别为142％和32％，8h时则进一步增高，分别为216％和85％。提示内毒素不仅刺激外周组织释放CGRP，而且还能通过某些机制激活背根神经节CGRPmRNA的转录，使CGRP合成增加，以作为CGRP大量释放的重要补充来源。
To investigate the changes of both CGRP release and synthesis during endotoxicosis, CGRP mRNA in thoracolumbar dorsal root ganglia (DRG) was determined by the semi-quantity RT-PCR and the CGRP levels in plasma were measured by RIA. The results showed that plasma CGRP, but not mRNA, began to increase 30 min after endotoxin (5 mg/kg, i. v. ) administration. On the other hand, the CGRP levels in plasma and CGRP mRNA in DRG were significantly increased by 142% and 32% respectively at the 3 h of injection and by 216% and 85% respectively at the 8 h of injection.The data suggest that not only the release of CGRP from the peripheral organs, but also the transcription of CGRP mRNA and synthesis of CGRP in sensory neurons are increased during the development of endotoxicosis in the rat. Presumably the DRG is an important source of CGRP release in the response to endotoxicosis
出处
《生理学报》
CAS
CSCD
北大核心
1997年第2期160-166,共7页
Acta Physiologica Sinica
基金
国家杰出青年基金!39425027
