摘要
本工作采用大鼠垂体前叶（AP）细胞原代培养方法，以3H－TdR掺入率反映细胞增殖水平，研究了IL－2对AP细胞增殖的影响。结果表明：（1）IL－2（10～500U／ml）明显促进雌性大鼠包括妊娠大鼠的AP细胞的增殖，而抑制雄性大鼠AP细胞的增殖。（2）雌性大鼠行卵巢切除（OVX）二周后，IL－2对其AP细胞增殖的影响反转为抑制效应；若在卵巢切除二周内，每日给予0VX大鼠皮下注射5μg苯甲酸雌二醇，IL-2的促增殖效应可基本恢复。（3）雄性大鼠行睾丸切除术2周后，IL－2对其AP细胞的抑制效应消失，但并不发生反转。上述结果说明，IL－2参与调节AP细胞的增殖活动，并且该调节活动受性激素等生理因素的影响。
The present study was to investigate whether IL-2 is involved in the proliferation control of anterior pituitary (AP) cells. Primary culture of AP cells was obtained from either male or female Sprague-Dawley rats. The effects of IL-2 were assessed by 3H-thymidine incorporation rate. It was found that: (1) IL-2 (10~500 U/ml) significantly stimulated 3H-TdR incorporation into AP cells of female pregnant as well as none-pregnant rats, but inhibited 3H-TdR incorporation in the male rats. (2) The stimulatory effect of IL-2 was reversed in overiectomized rats, but could be restored when the overiectomized rats were treated with estradiol benzoate (5 μg/d per rat J. (3)When the male rats were orchiectomized for 2 weeks, the inhibitory effects of IL-2 did not occur. The above data indicated that the proliferative effect of IL-2 on AP is a joint action of sex steroids hormones.
出处
《生理学报》
CAS
CSCD
北大核心
1997年第2期204-210,共7页
Acta Physiologica Sinica
基金
国家自然科学基金!39670281
关键词
垂体前叶
细胞增殖
白细胞介素-2
interleukin-2
anterior pituitary
cell growth
3H-TdR incorporation
estradiol
rat
