摘要
目的探讨氟中毒大鼠肝、肾、脑组织抗氧化酶类及脂质过氧化物的变化以及维生素 C、E 单独和联合干预以及不同剂量干预对高氟状态下大鼠抗氧化酶活性及脂质过氧化物的影响。方法将120只 Wistar 大鼠随机分为9绀,饮水染氟建立氟中毒大鼠模型,并灌胃给予维生素 C 和/或维生素 E;9月后处死大鼠取肝、肾、腑组织测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氧酶(CAT)活性和脂质过氧化物丙二醛(MDA)含量。结果染氟组大鼠肝、肾、脑组织 MDA 含量显著增加,肝和腑绀织 SOD、GSH-Px 和 CAT 活性以及肾组织 GSH-Px 活性均显著降低(P<0.05),而肾组织 SOD、CAT 活性无明显变化;维生素 C、E 可不同程度地增强 SOD、GSH-Px 和 CAT 活性,降低 MDA 水平;联合干预维生素 C、E 可明显拮抗氟诱导的脂质过氧化作用,显著增强大鼠肝组织 SOD、GSH-Px 和 CAT活性;维生素 C 低制量干预对肾脏 SOD 和 CAT 以及脑 GSH-Px 和 CAT 具有明显的保护作用;维生素 E 高剂量干预显著增加脑 SOD 活性。结论维生素 C 和维生素 E 在一定剂量范围内可有效拮抗过量氟导敛的脂质过氧化作用,对氟中毒大鼠肝、肾、脑组织有明显的保护作用。
Objective To study the effects of Vitamin C and Vitamin E on the antioxidant status and lipid peroxidation level in the liver,kidney and the brain of fluorosis rat . Methods 120 wistar were divided into 9 groups. The fluorosis animal model were produced with high fluoride drinking water , and orally perfused jointly with VitC and/or VitE. The activities of superoxide dismutase（SOD） ,glutathione peroxidase （GSH- Px ） , Catalase（CAT） and the contents of maleic dialdehyde （MDA）in the liver,kidney and the brain were measured respectively by test kit. Results The SOD, GSH -Px and CAT contents were lower while the MDA contents higher in the fluoride exposed group（ P 〈0.05） . Correspondingly , the SOD, GSH -Px and CAT contents were higher , while the MDA contents lower in the groups added with various dose of VitaminC or Vitamin E（ abbreviated by Vitamin C or VitaminE respectively ＋ NaF） and the groups united supplementation of VitaminC and Vitamin E（ abbreviated by VitaminC andVitaminE respectively ＋ NaF） （ P 〈0.05 ）. Conclusions A specific amounts of Vitamin C anti Vitamin E may counteract the lipid peroxidation caused by fluoride intoxication and protects the liver,kidney and the brain tissues on the fluorosis rats.
出处
《中国地方病防治》
北大核心
2008年第2期93-97,共5页
Chinese Journal of Control of Endemic Diseases
基金
新疆维吾尔自治区高校科研计划青年启动基金项目(XJEDU2004S17)
