摘要
目的:观察前列地尔(PGE1)预处理对异丙肾上腺素(ISO)诱导的急性心肌缺血损伤大鼠心电图和血清心肌酶的影响。方法:45只Wistar大鼠随机分为3组,即正常对照组、模型损伤组、PGE1治疗组,每组15只。正常对照组不给予任何处理;模型损伤组、PGE1治疗组皮下注射ISO10mg/kg复制大鼠急性心肌损伤模型;PGE1治疗组预先给予尾静脉注射PGE1,剂量为5μg·kg-1·d-1,每天1次,连续3d,于末次给药24h后皮下注射10mg/kgISO。24h后记录各组心电图变化,测定并比较血清乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)水平。结果:与正常对照组相比,模型损伤组大鼠心电图ST段明显抬高,血清LDH、CK水平明显升高(P<0.01)。与模型损伤组相比,预先静脉注射PGE1可对抗ISO所致心肌缺血大鼠心电图ST段的异常抬高(P<0.05),并降低血清LDH和CK的水平(P<0.01)。结论:PGE1可有效抑制急性心肌缺血损伤大鼠ST段的抬高和调节心肌酶活性,保护心肌细胞膜结构完整性,从而保护缺血损伤心肌。
Objective. To observe the effects of Prostaglandin E1 （PGE1） on electrocardiogram and cardiac enzymes of myocardial ischemic injury induced by isoproterenoe （ISO） in rats. Methods： Forty-five Wistar rats were randomly divided into 3 groups as following （n = 15） ： normal control group, ISO group and PGE1 group. The rats in normal control group without any treatment. The rats in ISO group were sc ISO （10 mg/kg） to prepare the model of acute ischemic myocardium. Rats in PGE1 group were injected PGE1 （5μg·kg^-1·d^-1） by coccygeal vein continuously for 3 days, then gave them identical dose ISO as before. 24 hour after the last treatment, recording the changes of ECG, measuring serum lactic dehydrogenase （LDH）, creatin kinase （CK） content. Results： Compared with normal control group, serum LDH, CK activities in ISO group were significantly increased （P 〈0.01）. Compared with ISO group, intravenous injection of PGE1 decreased the elevation of ST-T period induced by ISO remarkably （P 〈0.05） ; reduced serum LDH and CK contents （P 〈0.01）. Conclusion： PGE1 can inhibit the elevation of ST-T period of acute myocardial ischemia rats, and regulate the activity of cardiac enzymes to protect the ischemic myocardium.
出处
《新疆医科大学学报》
CAS
2008年第2期171-173,共3页
Journal of Xinjiang Medical University
关键词
心肌缺血
异丙肾上腺素
前列地尔
myocardial ischemia
isoproterenol
prostaglandin E1
