摘要
研究低分子量地黄多糖(LRPS)对p53基因表达的影响.方法:利用定量PCR的方法检测p53基因表达的水平.结果:LRPS在最适抗肿瘤有效剂量20和40mg·kg-1条件下,Lewis肺癌组织内p53基因的表达水平分别是152和148,对照组为046,环磷酰胺组为160.LRPS能使Lewis肺癌组织内p53基因的表达明显增加.结论:LRPS对抗癌基因p53表达的影响是其抗肿瘤作用的机制之一.
AIM: To study the effect of low molecular weight Rehmannia glutinosa polysaccharides (LRPS) on p53 gene expression. METHODS: The level of p53 gene expression was determined by quantitative polymerase chain reaction and autoradiography. RESULTS: The levels of p53 mRNA in Lewis lung cancer tissue were 0 46, 1 52, 1 48, and 1 60 respectively after saline, LRPS 20 and 40 mg·kg -1 , and cyclophosphamide 10 mg·kg -1 , which were the effective doses inhibiting the growth of tumor tissue cells in vivo . LRPS markedly increased p53 gene expression. CONCLUSION: LRPS effect on p53 gene expression is one of the mechanisms of antitumor activity.
出处
《中国药理学报》
CSCD
1997年第5期471-474,共4页
Acta Pharmacologica Sinica
关键词
地黄
多糖
P53基因
聚合酶链反应
Rehmannia glutinosa
polysaccharides
cyclophosphamide
phytogenic antineoplastic agents
lung neoplasms
p53 genes
polymerase chain reaction
autoradiography